Superior field performance of waxy corn engineered using CRISPR-Cas9
Superior field performance of waxy corn engineered using CRISPR-Cas9
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DOI：
10.1038/s41587-020-0444-0
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发表时间：
2020-03-09
影响因子：
46.9
通讯作者：
Meeley, R. Bob
中科院分区：
文献类型：
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作者：
Gao, Huirong;Gadlage, Mark J.;Meeley, R. Bob
我们通过CRISPR-Cas9编辑12个优良自交系的糯性等位基因，创造了糯性玉米杂交种，这一过程比采用回交和标记辅助选择的传统性状导入快了一年多。25个地点的田间试验表明，CRISPR-糯玉米杂交种的农艺性状优于导入杂交种，平均每亩增产5.5蒲式耳。大田试验表明，经过基因编辑的糯玉米品系的产量高于传统性状导入杂交种。
We created waxy corn hybrids by CRISPR-Cas9 editing of a waxy allele in 12 elite inbred maize lines, a process that was more than a year faster than conventional trait introgression using backcrossing and marker-assisted selection. Field trials at 25 locations showed that CRISPR-waxy hybrids were agronomically superior to introgressed hybrids, producing on average 5.5 bushels per acre higher yield.Gene-edited waxy corn lines have higher yields in field trials than hybrids produced by traditional trait introgression.