Two-Photon imaging of DiO-labelled Meissner corpuscle in living mouse's fingertip
Two-Photon imaging of DiO-labelled Meissner corpuscle in living mouse's fingertip
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活体小鼠指尖 DiO 标记迈斯纳小体的双光子成像
DOI：
10.1109/toh.2016.2574718
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发表时间：
2016
期刊：
影响因子：
--
通讯作者：
Miyata T
中科院分区：
文献类型：
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作者：
Pham TQ;Hoshi T;Tanaka Y;Sano A;Kawaue T;Miyata T
迈斯纳小体是一种快速适应的I型（FA-I）机械受体，位于皮肤的真皮乳头上。迈斯纳小体以其复杂的结构而闻名，由螺旋轴突、板层细胞和胶原蛋白胶囊组成。荧光显微镜已成为观察迈斯纳小体及其内部结构的一种方便的方法。这种方法需要用指尖横截面制备样品并在观察前进行抗体染色。各种显微镜可用于观察，如共聚焦显微镜，透射电子显微镜（TEM），或扫描电子显微镜（SEM）。虽然迈斯纳小体的解剖形状、分布和组成是公认的，但它们大多是从固定组织的观察中确定的。因此，由于缺乏体内实验和样本之间的个体差异，对机械转导的了解受到限制。在这项研究中，我们提出了一种新的微创成像方法，该方法结合了亲脂性碳氰胺染色技术和双光子显微镜。这种结合使我们能够在活老鼠体内反复观察迈斯纳小体。
Meissner corpuscles are the fast adapting type I (FA-I) mechanoreceptor that locates at the dermal papillae of skin. The Meissner corpuscle is well known for its complex structure, consisting of spiral axons, lamellar cells, and a collagen capsule. Fluorescent microscopy has become a convenient method for observing the Meissner corpuscle and its inner structure. This method requires preparing samples with fingertip cross-sections and performing antibody staining before observation. Various kinds of microscopy can be used for observation, such as confocal microscopy, transmission electron microscopy (TEM), or scanning electron microscopy (SEM). Although the anatomical shape, distribution, and components of Meissner corpuscle are recognized, they have been mostly determined from observations of fixed tissues. Therefore, knowledge of mechanical transduction is limited by the lack of in vivo experiments and individual differences among samples. In this study, we propose a novel less invasive imaging method that incorporates a staining technique with lipophilic carbocyanineand two-photon microscopy. This combination allows us to repetitively observe the Meissner corpuscle in a living mouse.