Expression of the 27-kDa heat shock protein (HSP27) in gastric carcinomas and adjacent normal, metaplastic, and dysplastic gastric mucosa, and its prognostic significance
Expression of the 27-kDa heat shock protein (HSP27) in gastric carcinomas and adjacent normal, metaplastic, and dysplastic gastric mucosa, and its prognostic significance
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DOI：
10.1007/s00432-002-0357-y
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发表时间：
2002-08-01
影响因子：
3.6
通讯作者：
Papavassiliou, AG
中科院分区：
文献类型：
--
作者：
Kapranos, N;Kominea, A;Papavassiliou, AG
目的：热休克蛋白27（HSP 27）在胃癌和癌旁正常、化生和异型增生胃粘膜中的表达及其与临床病理参数和患者生存的相关性研究。方法：采用免疫组织化学方法对福尔马林固定的石蜡包埋切片进行HSP 27单克隆抗体染色。对86例胃癌组织及癌旁正常、化生和异型增生胃粘膜中HSP 27的表达进行了检测和比较。结果如下：在正常胃粘膜中，HSP 27在86例中的68例（79%）中被检测到，并且在胃小凹的表面和上三分之二处更强。在异型增生胃粘膜中，HSP 27的免疫反应性通常高于邻近的正常上皮，并与异型增生的严重程度平行。HSP 27阳性表达率为62.7%（54/86），且与淋巴结转移有关（P = 0.03）。HSP 27在晚期肿瘤和女性患者中的表达也较高。在单因素分析中，HSP 27表达与总生存期缩短相关（P = 0.04），但在多因素分析中，这种关系并不存在。结论：我们的研究结果表明：i）HSP 27通常在正常胃上皮中表达，其中它似乎发挥保护作用;和ii）HSP 27参与胃癌发生，并且它的表达似乎与不利预后和较短总体存活的参数相关。
Purpose: The investigation of heat shock protein 27 (HSP27) expression in gastric cancer and adjacent normal, metaplastic, and dysplastic gastric mucosa and its correlation with clinicopathological parameters and survival of patients. Methods: Immunohistochemical methodology was performed on formalin-fixed paraffin-embedded sections by using a monoclonal anti-HSP27 antibody. HSP27 expression was screened and compared in 86 cases of gastric carcinoma and adjacent normal, metaplastic, and dysplastic gastric mucosa. Results: In the normal mucosa, HSP27 was detected in 68 out of 86 cases (79%) and was more intense in the surface and upper two-thirds of gastric foveolae. In dysplastic gastric mucosa, HSP27 immunoreactivity was usually higher than that of the adjacent normal epithelium and was parallel to the severity of dysplasia. HSP27 expression was found in 54 out of 86 (62.7%) gastric carcinomas and was significantly related to more than six metastatic lymph nodes (P = 0.03). HSP27 expression was also higher in tumors of advanced stage and in those of female patients. HSP27 expression was associated with shorter overall survival in univariate analysis (P = 0.04), but this relationship was not retained in multivariate analysis. Conclusions: Our findings indicate that: i) HSP27 is commonly expressed in normal gastric epithelium where it seems to exert a protective role; and ii) HSP27 is involved in gastric carcinogenesis and its expression appears to be associated with parameters of unfavorable prognosis and shorter overall survival.