Characterization of the cis-acting elements of the mouse myelin P2 promoter.
Characterization of the cis-acting elements of the mouse myelin P2 promoter.
复制标题
小鼠髓磷脂 P2 启动子顺式作用元件的表征。
DOI：
10.1002/jnr.490360503
复制
发表时间：
1993
影响因子：
4.2
通讯作者：
Tennekoon,GI
中科院分区：
文献类型：
--
作者：
Bharucha,VA;Peden,KW;Subach,BR;Narayanan,V;Tennekoon,GI
髓鞘P2是一种碱性蛋白质，表观分子量为14，800。在周围神经系统中，P2主要表达于雪旺细胞的胞浆中。虽然P2的功能尚不清楚，但其与脂肪酸结合蛋白家族的惊人同源性使人们认为P2可能作为脂肪酸转运分子发挥作用。为了研究控制P2表达的DNA元件，将编码区的5′端序列克隆到报告基因的上游。构建了一系列3′和5′启动子突变体，并在转染到第二代雪旺细胞和MT4 H1雪旺细胞系中后测定了它们的活性。使用这种策略，我们已经确定了一个217 bp的沉默区和一个142 bp的正调控区。此外，我们还定位了启动子中响应cAMP诱导和转录因子CCAAT/增强子结合蛋白（C/EBP）的5′侧翼序列。© 1993 Wiley利斯公司
Myelin P2is a basic protein of an apparent molecular weight of 14,800. Expression of P2has been found largely in the cytosol of Schwann cells in the peripheral nervous system. Although the function of P2is unknown, its striking homology to a family of fatty acid binding proteins has led to the idea that P2may function as a fatty acid transport molecule. To investigate the DNA elements that control the expression of P2, sequences 5′ to the coding region were cloned upstream of thecatreporter gene. A series of 3′ and 5′ promoter mutants was constructed and their activity determined following transfection into secondary Schwann cells and the MT4H1 Schwann cell line. Using this strategy, we have identified a 217 bp silencer region and a 142 bp positive regulatory region. In addition, we have localized the 5′ flanking sequences in the promoter that are responsive to cAMP induction and to the transcription factor CCAAT/enhancer binding protein (C/EBP). © 1993 Wiley‐Liss, Inc.