Mass Spectrometry Applied to Bottom-Up Proteomics: Entering the High-Throughput Era for Hypothesis Testing
Mass Spectrometry Applied to Bottom-Up Proteomics: Entering the High-Throughput Era for Hypothesis Testing
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DOI：
10.1146/annurev-anchem-071015-041535
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发表时间：
2016-01-01
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影响因子：
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通讯作者：
Aebersold, Ruedi
中科院分区：
文献类型：
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作者：
Gillet, Ludovic C.;Leitner, Alexander;Aebersold, Ruedi
蛋白质构成一类关键的分子成分，在活细胞中执行重要的生化反应。无论目的是广泛表征给定蛋白质，还是对样品的蛋白质组含量进行高通量定性和定量分析，液相色谱与串联质谱联用已成为首选技术。在这篇综述中，我们总结了应用于自下而上蛋白质组学的质谱分析的现状，该方法侧重于分析从蛋白质水解消化中获得的肽。随着仪器和方法的最新进展，我们表明该领域正在从提供一长串蛋白质的定性鉴定转向为大量样品中的大量蛋白质提供高度一致和准确的定量值。我们相信这一转变将对蛋白质组学和生命科学研究领域产生深远的影响。
Proteins constitute a key class of molecular components that perform essential biochemical reactions in living cells. Whether the aim is to extensively characterize a given protein or to perform high-throughput qualitative and quantitative analysis of the proteome content of a sample, liquid chromatography coupled to tandem mass spectrometry has become the technology of choice. In this review, we summarize the current state of mass spectrometry applied to bottom-up proteomics, the approach that focuses on analyzing peptides obtained from proteolytic digestion of proteins. With the recent advances in instrumentation and methodology, we show that the field is moving away from providing qualitative identification of long lists of proteins to delivering highly consistent and accurate quantification values for large numbers of proteins across large numbers of samples. We believe that this shift will have a profound impact for the field of proteomics and life science research in general.