A MYB-Related Transcription Factor from Lilium lancifolium L. (LlMYB3) Is Involved in Anthocyanin Biosynthesis Pathway and Enhances Multiple Abiotic Stress Tolerance in Arabidopsis thaliana
A MYB-Related Transcription Factor from Lilium lancifolium L. (LlMYB3) Is Involved in Anthocyanin Biosynthesis Pathway and Enhances Multiple Abiotic Stress Tolerance in Arabidopsis thaliana
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百合 (Lilium lancifolium L.) 的 MYB 相关转录因子 (LlMYB3) 参与拟南芥花青素生物合成途径并增强多种非生物胁迫耐受性
DOI：
10.3390/ijms20133195
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发表时间：
2019
影响因子：
5.6
通讯作者：
Yingmin Lyu
中科院分区：
文献类型：
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作者：
Yubing Yong;Yue Zhang;Yingmin Lyu
大多数商业百合品种对非生物胁迫比较敏感。然而，卷丹（Lilium lancifolium L.），是亚洲分布最广的野生百合之一，具有较强的抗非生物胁迫能力。因此，筛选出百合逆境响应候选基因对提高植物的抗逆性具有重要意义。在本研究中，一个MYB相关的同源物（LlMYB 3）从老虎百合功能的特点是作为一个积极的调节植物的抗逆性。LlMYB 3是一种在C-末端具有转录激活活性的核蛋白。LlMYB 3基因的表达受多重胁迫诱导。几种胁迫相关的顺式作用调控元件（MYBRS、MYCRS、LTRE和DRE/CRT）位于LlMYB 3的启动子内;然而，启动子活性不能被各种胁迫处理充分诱导。LlMYB 3基因在拟南芥中的高效表达转基因植株表现出阿坝敏感性，并增强了对寒冷、干旱和盐胁迫的耐受性。此外，我们发现LlMYB 3与LlCHS 2基因在冷处理下高度共表达;酵母单杂交（Y1 H）分析表明LlMYB 3能够结合LlCHS 2的启动子。这些结果表明，胁迫响应LlMYB 3可能参与花色素苷的生物合成途径，调节百合的抗逆性。
Most commercial cultivars of lily are sensitive to abiotic stresses. However, tiger lily (Lilium lancifolium L.), one of the most widely distributed wild lilies in Asia, has strong abiotic stresses resistance. Thus, it is indispensable to identify stress-responsive candidate genes in tiger lily for the stress resistance improvement of plants. In this study, a MYB related homolog (LlMYB3) from tiger lily was functionally characterized as a positive regulator in plant stress tolerance. LlMYB3 is a nuclear protein with transcriptional activation activity at C-terminus. The expression of LlMYB3 gene was induced by multiple stress treatments. Several stress-related cis-acting regulatory elements (MYBRS, MYCRS, LTRE and DRE/CRT) were located within the promoter of LlMYB3; however, the promoter activity was not induced sufficiently by various stresses treatments. Overexpressing LlMYB3 in Arabidopsis thaliana L. transgenic plants showed ABA hypersensitivity and enhanced tolerance to cold, drought, and salt stresses. Furthermore, we found LlMYB3 highly co-expressed with LlCHS2 gene under cold treatment; yeast one-hybrid (Y1H) assays demonstrated LlMYB3 was able to bind to the promoter of LlCHS2. These findings suggest that the stress-responsive LlMYB3 may be involved in anthocyanin biosynthesis pathway to regulate stress tolerance of tiger lily.