A novel multimodal optical imaging system for early detection of oral cancer.
A novel multimodal optical imaging system for early detection of oral cancer.
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DOI：
10.1016/j.oooo.2015.10.020
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发表时间：
2016-03
期刊：
影响因子：
--
通讯作者：
Maitland KC
中科院分区：
文献类型：
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作者：
Malik BH;Jabbour JM;Cheng S;Cuenca R;Cheng YS;Wright JM;Jo JA;Maitland KC
几种成像技术已被提倡作为临床辅助手段，以提高可疑口腔病变的识别能力。然而，与传统的口腔检查技术相比，这些技术还没有显示出更好的敏感性或特异性。我们开发了一种多模式、多尺度的光学成像系统，将宏观的荧光寿命成像(FLIM)和亚细胞形态成像的反射共聚焦显微镜(RCM)相结合，用于口腔癌的早期检测。我们在切除的人类口腔组织上测试了我们的系统。共对4个组织标本进行了成像。这些标本分别被诊断为：临床正常、口腔扁平苔藓、牙龈增生和表面浸润性鳞癌(SCC)。记录了每个样品的光学和荧光寿命特性。正常病变、良性病变和鳞癌病变之间的定量和定性差异均可通过Flim-RCM成像解决。结果表明，基于这两种方法的综合方法可以潜在地实现对大面积口腔上皮组织的快速筛选和评估。正在进行的人体口腔成像研究的早期结果说明了这两种模式的协同结合。一种基于口腔粘膜这种光学特征的辅助设备可能被用于检测口腔癌的早期阶段。
Several imaging techniques have been advocated as clinical adjuncts to improve identification of suspicious oral lesions. However, these have not yet shown superior sensitivity or specificity over conventional oral examination techniques. We developed a multimodal, multi-scale optical imaging system that combines macroscopic biochemical imaging of fluorescence lifetime imaging (FLIM) with subcellular morphologic imaging of reflectance confocal microscopy (RCM) for early detection of oral cancer. We tested our system on excised human oral tissues. A total of four tissue specimen were imaged. These specimens were diagnosed as one each: clinically normal, oral lichen planus, gingival hyperplasia, and superficially-invasive squamous cell carcinoma (SCC). The optical and fluorescence lifetime properties of each specimen were recorded. Both quantitative and qualitative differences between normal, benign and SCC lesions can be resolved with FLIM-RCM imaging. The results demonstrate that an integrated approach based on these two methods can potentially enable rapid screening and evaluation of large areas of oral epithelial tissue. Early results from ongoing studies of imaging human oral cavity illustrate the synergistic combination of the two modalities. An adjunct device based on such optical characterization of oral mucosa can potentially be used to detect oral carcinogenesis in early stages.