Amendment of Agricultural Soil with Metal Nanoparticles: Effects on Soil Enzyme Activity and Microbial Community Composition
Amendment of Agricultural Soil with Metal Nanoparticles: Effects on Soil Enzyme Activity and Microbial Community Composition
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金属纳米颗粒改良农业土壤对土壤酶活性和微生物群落组成的影响
DOI：
10.1021/acs.est.7b05389
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发表时间：
2018-02-20
影响因子：
11.4
通讯作者：
Tufenkji, Nathalie
中科院分区：
文献类型：
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作者：
Asadishad, Bahareh;Chahal, Shawninder;Tufenkji, Nathalie
人们正在考虑将几种类型的工程纳米粒子 (ENP) 直接应用于土壤，以减少农药的使用和降解、提供微量营养素、控制病原体并提高作物产量。本研究探讨了不同金属 ENP 及其溶解离子对生物固体改良农业土壤微生物群落组成和酶活性的影响。在用不同浓度（1、10 或 100 mg ENP/kg 土壤）银 (nAg)、氧化锌 (nZnO)、氧化铜 (nCuO) 或二氧化钛 (nTiO(2)) 纳米粒子及其离子处理 30 天的生物固体改良土壤中，测量了五种细胞外养分循环酶的活性。 30天时，nZnO和nCuO对土壤酶活性没有显着影响，或者增强了酶活性。相比之下，当以颗粒或溶解形式（100 mg/kg）给药时，Ag 会抑制选定的酶。 nTiO(2) 要么没有显着影响，要么略微降低了酶活性。 Illumina MiSeq 微生物群落测序表明，土壤微生物群落组成在暴露于高剂量金属离子或 nAg 时发生变化，而在 nTiO(2) 存在下变化可忽略不计。一些类群对 nAg 和 Agt 的反应不同。这项工作展示了金属 ENP 在引入生物固体改良的土壤后如何影响土壤酶活性和微生物群落组成，具体取决于其类型、浓度和溶解行为，从而为纳米技术在农业中的可持续应用提供了急需的信息。
Several types of engineered nanoparticles (ENPs) are being considered for direct application to soils to reduce the application and degradation of pesticides, provide micronutrients, control pathogens, and increase crop yields. This study examined the effects of different metal ENPs and their dissolved ions on the microbial community composition and enzyme activity of agricultural soil amended with biosolids. The activity of five extracellular nutrient-cycling enzymes was measured in biosolid-amended soils treated with different concentrations (1, 10, or 100 mg ENP/kg soil) of silver (nAg), zinc oxide (nZnO), copper oxide (nCuO), or titanium dioxide (nTiO(2)) nanoparticles and their ions over a 30-day period. At 30 days, nZnO and nCuO either had no significant effect on soil enzyme activity or enhanced enzyme activity. In contrast, Ag inhibited selected enzymes when dosed in particulate or dissolved form (at 100 mg/kg). nTiO(2) either had no significant effect or slightly decreased enzyme activity. Illumina MiSeq sequencing of microbial communities indicated a shift in soil microbial community composition upon exposure to high doses of metal ions or nAg and negligible shift in the presence of nTiO(2). Some taxa responded differently to nAg and Agt This work shows how metal ENPs can impact soil enzyme activity and microbial community composition upon introduction into soils amended with biosolids, depending on their type, concentration, and dissolution behavior, hence providing much needed information for the sustainable application of nanotechnology in agriculture.