Study of the anti-proliferative effects and synergy of phthalides from Angelica sinensis on colon cancer cells
Study of the anti-proliferative effects and synergy of phthalides from Angelica sinensis on colon cancer cells
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DOI：
10.1016/j.jep.2008.07.027
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发表时间：
2008-10-30
影响因子：
5.4
通讯作者：
Lin, Ge
中科院分区：
文献类型：
--
作者：
Kan, Winnie Lai Ting;Cho, Chi Hin;Lin, Ge
当归是一种治疗妇科和胃肠道疾病的中草药，也与癌症化疗有关。本研究的目的：在本研究中，研究了三种主要的当归酞，即正丁基苯酞（BLP），senkyunoprotein A（SKA）和Z-藁本内酯（LGT）对结肠癌HT-29细胞的细胞毒性和抗增殖作用，以及它们的协同作用。材料与方法：采用MTT法和[H-3]胸苷掺入法，分别检测其对人结肠癌HT-29细胞和正常结肠CCD-18 Co细胞的细胞毒活性和抗增殖活性。结果：三种苯酞类化合物剂量依赖性地降低HT-29细胞的存活率，其作用强于CCD-18 Co细胞。在HT-29细胞中，SKA、LGT和BLP抑制细胞增殖的IC 50值分别为54.17 +/- 5.10、60.63 +/- 6.79和236.90 +/- 18.22 μ M。结论：3种酞菁类化合物均具有一定的抗肿瘤作用，但与当归提取物中的其他成分联合使用时，其抗肿瘤作用更强。(C)2008爱思唯尔爱尔兰有限公司保留所有权利。
Angelica sinensis is a Chinese medicinal herb for treating gynecological and gastrointestinal disorders, and also in conjunction with cancer chemotherapy.Aim of the study: In the present study, the cytotoxic and anti-proliferative effects of three main Angelica sinensis phthalicles, namely n-butylidenephthalide (BLP), senkyunolide A (SKA) and z-ligustilide (LGT), and their synergy on colon cancer HT-29 cells were investigated. Moreover, the results obtained in both human colon cancer HT-29 and normal colon CCD-18Co cells were compared for the investigation of selectivity.Materials and methods: MTT and [H-3] thymidine incorporation assays were used for the evaluation of cytotoxic and anti-proliferative effects, respectively. Interactions among phthalicles were determined by median-effect analysis.Results: All three phthalides dose-dependently decreased cell viability more potently in HT-29 than in CCD-18Co cells. The IC50 values for inhibition of cell proliferation for SKA, LGT and BLP were 54.17 +/- 5.10, 60.63 +/- 6.79 and 236.90 +/- 18.22 mu M, respectively, in HT-29 cells. Angelica sinensis extract demonstrated significant synergy in inhibiting cell proliferation.Conclusions: The three phthalicles might have anti-cancer potential, yet the phthalicles, in combination with other ingredients in Angelica sinensis extract, display significant synergy leading to a stronger antitumor effect. (C) 2008 Elsevier Ireland Ltd. All rights reserved.