GENETIC-IMPROVEMENT OF TRICHODERMA-REESEI FOR LARGE-SCALE CELLULASE PRODUCTION
GENETIC-IMPROVEMENT OF TRICHODERMA-REESEI FOR LARGE-SCALE CELLULASE PRODUCTION
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DOI：
10.1016/0141-0229(88)90012-9
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发表时间：
1988-06-01
影响因子：
3.4
通讯作者：
TIRABY, G
中科院分区：
文献类型：
--
作者：
DURAND, H;CLANET, M;TIRABY, G
设计了一种基于平板筛选试验的策略，用于筛选适合于工业规模生产纤维素酶的里氏木霉（Trichoderma reesei）突变菌株（EC 3.2.1.4）。连续分离出6个突变体世代，每个世代都满足所有三个标准：（1）与前一个相比提高的生产力，（2）高稳定性，（3）进一步改进的能力。最终选择的突变体CL 847与起始菌株QM 9414相比，在纤维素培养基中纤维素酶生产率提高了四倍，对分解代谢物阻遏具有抗性，β- D-葡萄糖苷酶（EC 3.2.1.21）比活性，它是部分组成型的。在以乳糖或葡萄糖为唯一碳源的简单培养基中获得高纤维素酶浓度。该菌株目前用于工业发酵工厂。
A strategy based on plate screening tests was designed for the selection of mutant strains of the fungus Trichoderma reesei suitable for cellulase (EC 3.2.1.4) production on an industrial scale. Six mutant generations were successively isoalted, each of them fulfilling all of the three criteria: (1) improved productivity compared to the previous one, (2) high stability, (3) ability to be further improved. The mutant ultimately selected, CL 847, exhibited a four-fold inrease in cellulase productivity in cellulose media as compared to the starting strain QM 9414, resistance to catabolite repression, increased .beta.-D-glucosidase (EC 3.2.1.21) specific activity and it was partially constitutive. High cellulase concentrations were obtained in simple media with lactose or glucose as sole carbon source. The strain is currently used in an indutrial fermentation plant.