ANALYSIS OF THE CONSTANCY OF DNA SEQUENCES DURING DEVELOPMENT AND EVOLUTION OF THE NEMATODE CAENORHABDITIS-ELEGANS
ANALYSIS OF THE CONSTANCY OF DNA SEQUENCES DURING DEVELOPMENT AND EVOLUTION OF THE NEMATODE CAENORHABDITIS-ELEGANS
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DOI：
10.1073/pnas.76.3.1333
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发表时间：
1979-01-01
影响因子：
11.1
通讯作者：
HIRSH, D
中科院分区：
文献类型：
--
作者：
EMMONS, SW;KLASS, MR;HIRSH, D
研究了发育过程中DNA重排的发生和进化过程中DNA分歧的速率。从C.秀丽变种布里斯托（Bristol），从C.秀丽变种贝尔热拉克和C. Briggsae进行了比较。通过将放射性克隆片段与限制性内切酶杂交来检测限制性内切酶片段，所述限制性内切酶已在琼脂糖凝胶上按大小分级并转移到硝酸纤维素片上。以这种方式，观察并比较了大约50个BamHI限制性片段。精子和体细胞DNA的片段是相同的，15%的大小不同的两个菌株之间。两个种间的交叉同源性很小。如果C.在发育过程中，它们必须影响不到百分之几的限制性片段或限制性位点。2株菌与2种菌之间存在差异。是惊人的伟大。
The occurrence of rearrangements in DNA during development and the rate of DNA divergence during evolution were studied. DNA restriction fragments from sperm cells and somatic cells of C. elegans var. Bristol, from somatic tissues of C. elegans var. Bergerac and from C. briggsae were compared. Restriction fragments were detected by hybridizing radioactive cloned fragments to restriction digests that had been fractionated by size on agarose gels and transferred to nitrocellulose sheets. In this way, approximately 50 BamHI restriction fragments were visualized and compared. Fragments from sperm and somatic DNA were identical; 15% differed in size between the 2 strains. Little cross homology was found between the 2 spp. If rearrangements occur in C. elegans DNA during development, they must affect fewer than a few percent of the restriction fragments or restriction sites. The difference found between the 2 strains and the 2 spp. is surprisingly great.