INVITRO MODELS OF DIFFERENTIATED SERTOLI-CELL STRUCTURE AND FUNCTION
INVITRO MODELS OF DIFFERENTIATED SERTOLI-CELL STRUCTURE AND FUNCTION
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DOI：
10.1007/bf02629090
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发表时间：
1988-06-01
期刊：
影响因子：
--
通讯作者：
DYM, M
中科院分区：
文献类型：
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作者：
HADLEY, MA;BYERS, SW;DYM, M
维持在塑料培养容器上的常规培养物中的支持细胞的原代培养物不保留其体内对应物的许多结构和功能特性。通过将睾丸固有的某些环境参数并入支持细胞培养系统中，可以更好地维持支持细胞表型。这些环境参数包括a）高细胞密度，B）独特的细胞外基质，c）细胞基底质膜与睾丸中血液来源的营养物质之间的半渗透性支持，d）细胞顶面和底面的化学上不同的微环境，以及e）支持细胞和其他睾丸细胞类型之间的细胞间相互作用。使用三种不同的支持细胞培养，我们已经证明了这些环境参数中的每一个在获得更好的支持细胞培养模型的重要性。
Primary cultures of Sertoli cells maintained in conventional cultures on plastic culture vessels do not retain many of the structural and functional properties of their in vivo counterparts. Sertoli cell phenotype is better maintained by incorporating certain environmental parameters, intrinsic to the testic, into the Sertoli cell culture system. These environmental parameters include a) high cell density, b) a unique extracellular matrix, c) a semipermeable support between the basal plasma membrane of the cells and blood-derived nutrients in the interstitium, d) chemically distinct microenvironments at the apical and basal surfaces of the cells, and e) cell-to-cell interactions among Sertoli cells and other testicular cell types. Using three variations of Sertoli cell culture we have demonstrated the importance of each of these environmental parameters in obtaining a better Sertoli cell culture model.