Differential effects of flagellins from Pseudomonas syringae pv. tabaci, tomato and glycinea on plant defense response
Differential effects of flagellins from Pseudomonas syringae pv. tabaci, tomato and glycinea on plant defense response
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DOI：
10.1016/s0981-9428(02)00018-9
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发表时间：
2003-02-01
影响因子：
6.5
通讯作者：
Ichinose, Y
中科院分区：
文献类型：
--
作者：
Taguchi, F;Shimizu, R;Ichinose, Y
研究决定丁香假单胞菌 pv 之间不相容相互作用的因素。烟粉虱和非寄主植物中，通过 DEAE 柱层析从细菌培养的营养不良培养基的上清液中部分纯化过敏反应（HR）诱导子。激发子活性部分中的主要蛋白质被鉴定为鞭毛蛋白，它是鞭毛丝的组成部分。从丁香假单胞菌 pv. 中纯化的鞭毛蛋白。番茄和大豆是烟草植物的不相容病原体，可诱导烟草（Nicotiana tabacum）亮黄（BY-2）细胞中染色体 DNA 断裂和氧化爆发，并伴有程序性细胞死亡，但来自 pv. 番茄和大豆的鞭毛蛋白。烟粉虱是一种兼容的病原体，但没有。然而，从fliC基因推导的鞭毛蛋白的氨基酸序列在这些丁香假单胞菌致病变种之间表现出高度同源性。特别地，pv的氨基酸序列。烟粉虱和甘氨酸完全相同。然而，大肠杆菌中产生的两种重组鞭毛蛋白在 BY-2 细胞中都具有 HR 诱导活性。这些结果表明鞭毛蛋白的翻译后修饰对于烟草细胞中 HR 诱导能力具有重要作用。此外，我们讨论了烟草细胞上鞭毛蛋白不同诱导子活性的原因以及鞭毛蛋白在确定特异性中的作用。 (C) 2002 年科学与医学版 Elsevier SAS。版权所有。
To investigate the factor that determines incompatible interactions between Pseudomonas syringae pv. tabaci and non-host plants, an elicitor of hypersensitive reaction (HR) was partially purified from the supernatant of a nutrient-poor medium of bacterial culture by DEAE column chromatography. The major protein in the elicitor-active fractions was identified as a flagellin which is a component of flagellar filaments. The flagellins purified from P. syringae pv. tomato and glycinea, incompatible pathogens of tobacco plants, induced fragmentation of chromosomal DNA and oxidative burst accompanied by programmed cell death in tobacco (Nicotiana tabacum) Bright Yellow (BY-2) cells, but the flagellin from pv. tabaci, a compatible pathogen, did not. However, the amino acid sequences of flagellins deduced from fliC genes showed a high homology among these P. syringae pathovars. In particular, the amino acid sequences of pv. tabaci and glycinea are completely identical. However, both recombinant flagellins produced in Escherichia coli possess HR-inducing activity in BY-2 cells. These results indicate that the post-translational modification of flagellins has an important role for HR-inducing ability in tobacco cells. Furthermore, we discuss the cause of a different elicitor activity among flagellins on tobacco cells and the role of flagellins in the determining specificity. (C) 2002 Editions scientifiques et medicales Elsevier SAS. All rights reserved.