Calcium waves propagate through radial glial cells and modulate proliferation in the developing neocortex
Calcium waves propagate through radial glial cells and modulate proliferation in the developing neocortex
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DOI：
10.1016/j.neuron.2004.08.015
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发表时间：
2004-09-02
期刊：
影响因子：
16.2
通讯作者：
Kriegstein, AR
中科院分区：
文献类型：
--
作者：
Weissman, TA;Riquelme, PA;Kriegstein, AR
成人新皮层中的大多数神经元是在短暂而强烈的神经元增殖期间胚胎产生的。放射状胶质细胞是一种瞬态胚胎细胞类型，因其在神经元迁移中起关键作用而闻名，最近被证明具有神经元祖细胞的功能，似乎产生了大多数皮层锥体神经元。因此，放射状胶质细胞调节可以影响神经元的产生、神经元的迁移和整个皮层结构；然而，放射状胶质细胞间的信号传导机制尚未得到直接研究。我们在这里证明钙波通过增生性皮质心室区（VZ）的放射状胶质细胞传播。放射状胶质钙波自发发生，需要连接蛋白半通道、P2Y(1) ATP受体和细胞内ip3介导的钙释放。此外，我们还发现，在胚胎神经发生的高峰期，波的破坏会减少VZ的增殖。综上所述，这些结果表明放射状胶质信号机制可能调节皮层神经元的产生。
The majority of neurons in the adult neocortex are produced embryonically during a brief but intense period of neuronal proliferation. The radial glial cell, a transient embryonic cell type known for its crucial role in neuronal migration, has recently been shown to function as a neuronal progenitor cell and appears to produce most cortical pyramidal neurons. Radial glial cell modulation could thus affect neuron production, neuronal migration, and overall cortical architecture; however, signaling mechanisms among radial glia have not been studied directly. We demonstrate here that calcium waves propagate through radial glial cells in the proliferative cortical ventricular zone (VZ). Radial glial calcium waves occur spontaneously and require connexin hemichannels, P2Y(1) ATP receptors, and intracellular IP3-mediated calcium release. Furthermore, we show that wave disruption decreases VZ proliferation during the peak of embryonic neurogenesis. Taken together, these results demonstrate a radial glial signaling mechanism that may regulate cortical neuronal production.