SPECIMEN PREPARATION FOR ELECTRON-MICROSCOPY USING LOW-TEMPERATURE EMBEDDING RESINS
SPECIMEN PREPARATION FOR ELECTRON-MICROSCOPY USING LOW-TEMPERATURE EMBEDDING RESINS
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DOI：
10.1111/j.1365-2818.1982.tb00358.x
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发表时间：
1982-01-01
影响因子：
2
通讯作者：
VILLIGER, W
中科院分区：
文献类型：
--
作者：
ARMBRUSTER, BL;CARLEMALM, E;VILLIGER, W
介绍了适合低温包埋的极性和非极性甲基丙烯酸酯树脂(Lowicryl K4M和HM20)的研究。首次将该系统应用于戊二醛固定、醋酸铀酰染色的细菌、线粒体和细胞-细胞接触区的各种膜结构。[本研究包括小鼠肝细胞、大肠杆菌和果蝇睾丸。]。
Studies using polar and non-polar methacrylate-based resins (Lowicryl K4M and HM20) suitable for low temperature embedding are described. The 1st applications of the system to various membrane structures in glutaraldehyde-fixed, uranyl acetate-stained thin sections of bacteria, mitochondria and cell-cell contact regions is presented. [Included in this study, were mouse hepatocytes, Escherichia coli and Drosophila melanogaster testes.].