Sequential changes in laminin and type IV collagen in the infarct zone - Immunohistochemical study in rat myocardial infarction
Sequential changes in laminin and type IV collagen in the infarct zone - Immunohistochemical study in rat myocardial infarction
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DOI：
10.1253/jcj.60.108
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发表时间：
1996-02-01
期刊：
影响因子：
--
通讯作者：
Tsuji, T
中科院分区：
文献类型：
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作者：
Morishita, N;Kusachi, S;Tsuji, T
背景：心肌梗死的愈合过程是影响心肌梗死预后的重要因素，影响心室重构。我们假设层粘连蛋白和IV型胶原在心肌梗死后的愈合过程中有助于细胞外基质的组装。我们检测了大鼠实验性心肌梗死后这两种成分的时序变化。材料和方法：在永久性左冠状动脉结扎后1d至10周内摘除大鼠心脏。用抗层粘连蛋白和IV型胶原的多克隆抗体进行免疫组织化学染色。结果：层粘连蛋白：3d时，层粘连蛋白在梗死区周围的肉芽组织中呈波浪状分布，不局限于细胞膜；外周区的染色分布逐渐增多，在第7~11d达到最大。分布1~2d后由梗死灶周围区向梗死灶中心区外扩散，2周后达中心点。达到这一最大值后，染色分布范围逐渐减小，但染色并未完全消失。IV型胶原：IV型胶原的变化与层粘连蛋白的变化基本相同。从第3天开始，脑梗塞周边区出现IV型胶原的波状染色！在第7-11天达到最大值，此后逐渐下降。结论：层粘连蛋白和IV型胶原在心肌梗死后较早参与梗死区细胞外基质的形成。
Background: The healing process, which affects ventricular remodeling, is an important factor in the prognosis of myocardial infarction. We hypothesized that laminin and type IV collagen contribute to extracellular matrix assembly in healing after myocardial infarction. We examined sequential changes in these two components after experimental myocardial infarction in rats.Materials and Methods: Hearts were excised from 1 day to 10 weeks after permanent left coronary ligation in rats. Immunohistochemical staining with a polyclonal antibody to laminin and type IV collagen was performed by the avidin-biotin-peroxidase method.Results: Laminin: On day 3, laminin initially appeared in a wavy fashion in the granulation tissue of the infarct peripheral zone and was not restricted to the cell membrane; the staining distribution in the peripheral zone then gradually increased, reaching a maximum on days 7-11. The distribution progressed from the peripheral zone to the outer lesion of the central zone of the infarct for 1-2 days, and reached the center point after 2 weeks. The extent of the staining distribution gradually decreased after reaching this maximum, but the staining did not completely disappear. Type IV Collagen: Changes in type IV collagen were essentially the same as those in laminin. A wavy staining pattern of type IV collagen appeared in the infarct peripheral zone from day 3! reached its maximum extent on days 7-11, and decreased gradually thereafter. The distribution progressed from the peripheral zone to the outer lesion of the central zone for 1-2 days, reaching the center point after 2 weeks.Conclusion: Laminin and type IV collagen contribute to extracellular matrix formation in the infarct zone relatively early after myocardial infarction.