Extracellular signal-regulated kinase 2 mRNA expression in the rat brain during aging.
Extracellular signal-regulated kinase 2 mRNA expression in the rat brain during aging.
复制标题
衰老过程中大鼠脑中细胞外信号调节激酶 2 mRNA 的表达。
DOI：
10.1023/a:1024948532633
复制
发表时间：
2003
影响因子：
4.4
通讯作者：
Sun,GraceY
中科院分区：
文献类型：
--
作者：
Simonyi,Agnes;Murch,Kevin;Sun,GraceY
衰老伴随着记忆和认知功能的丧失。细胞外信号调节激酶 (ERK) 通路已被证明在突触可塑性和记忆中发挥重要作用。尽管在老年大鼠的大脑皮层中发现基础 ERK1/2 活性降低，但尚未描述衰老过程中 ERK1/2 mRNA 表达的变化。在本研究中，我们利用定量原位杂交研究了雄性 Fisher 344 大鼠（三个年龄组）不同脑区 ERK2 mRNA 表达的年龄差异。在小脑或皮质区域未检测到 ERK2 mRNA 随年龄变化的变化。然而，在海马体中，与 3 个月大的组相比，12 个月大的组中 CA3 区域的 mRNA 水平下降了 20%。这些结果表明，衰老过程中观察到的 ERK1/2 活性受损可能并未在基因表达水平上受到调节。
Aging is accompanied by the loss of memory and cognitive functions. The extracellular signal-regulated kinase (ERK) pathway has been shown to play an essential role in synaptic plasticity and memory. Although a reduction in basal ERK1/2 activity has been found in the cerebral cortex in aged rats, changes in ERK1/2 mRNA expression during aging have not been described. In this study, we investigated age differences in the mRNA expression of ERK2 in different brain regions of male Fisher 344 rats (three age-groups) using quantitative in situ hybridization. No age-dependent changes in ERK2 mRNA were detected in the cerebellum or cortical areas. However, in the hippocampus, a 20% decline in mRNA levels was observed in the CA3 region in the 12-month-old group as compared to the 3-month-old group. These results suggest that the impairment in ERK1/2 activity observed during aging is probably not regulated at the gene expression level.