[Immunohistochemical study of glial cytoplasmic inclusion in multiple system atrophy].
[Immunohistochemical study of glial cytoplasmic inclusion in multiple system atrophy].
复制标题
多系统萎缩中胶质细胞质包涵体的免疫组织化学研究
DOI：
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发表时间：
1991
期刊：
影响因子：
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通讯作者：
R. Oue
中科院分区：
文献类型：
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作者：
N. Arai;M. Nishimura;M. Oda;Y. Morimatsu;R. Oue
近年来，在多系统萎缩患者的少突胶质细胞中，银染证实胶质细胞胞质包涵体(GCI)为嗜银细胞质。我们在所有20例多系统萎缩的病例中观察到这样的GCI。6例遗传性脊髓小脑变性患者均未发现GCI。免疫组织化学显示，GCI与泛素、α-微管蛋白和β-微管蛋白抗体呈阳性反应，其特征与以前的报道一致。此外，GCI首次被证明与微管相关蛋白-1B(5)的抗体发生反应，微管相关蛋白-1B(5)是细胞骨架组织的蛋白质之一，也是微管组装之间的交叉桥梁的组成部分。提示OCI的形成与MAP-1B(5)的免疫组织化学表达密切相关。
Recently, glial cytoplasmic inclusion (GCI) has been demonstrated to be argyrophilic cytoplasmic body by silver staining in the oligodendroglia of patients with multiple system atrophy. We observed such GCIs in all 20 cases of multiple system atrophy. No GCI was noticed in all 6 cases of hereditary spinocerebellar degenerations. Immunohistochemically, GCI was stained positively with antibodies to ubiquitin, alpha-tubulin, and beta-tubulin, of which characteristics is consistent with previous reports. In addition, GCI was first demonstrated to react with an antibody to microtubule-associated protein-1B(5), which is one of the proteins of cytoskeleton organization and a component of cross-bridges between microtubular assembly. The result suggests strong relationship between the formation of the OCI and immunohistochemical expression of MAP-1B(5).