The Sonic Hedgehog signaling pathway regulates inferior alveolar nerve regeneration
The Sonic Hedgehog signaling pathway regulates inferior alveolar nerve regeneration
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DOI：
10.1016/j.neulet.2017.12.051
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发表时间：
2018-04
影响因子：
2.5
通讯作者：
Yurie Yamada;A. Ohazama;T. Maeda;K. Seo
中科院分区：
文献类型：
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作者：
Yurie Yamada;A. Ohazama;T. Maeda;K. Seo
已知在外周神经如坐骨神经损伤后观察到Shh信号传导的激活。然而，Shh信号在周围神经再生过程中的确切作用尚未完全了解。下牙槽神经（IAN）是口腔手术中最常见的损伤。与坐骨神经不同，IAN与其他颅面组织分离，因为它位于下颌骨内的长骨管中。因此，IAN是研究周围神经再生的良好实验模型。在这项研究中，Shh信号在周围神经再生的作用进行了研究，使用小鼠IAN横断模型。在再生过程中，Shh信号被激活在整个远端区域的IAN和近端树桩。在横断部位应用环巴胺抑制Shh信号传导导致轴突在随机方向上异常生长，巨噬细胞数量减少，远端区域内髓鞘碎片增加。因此，Shh信号传导通过调节髓鞘降解参与外周神经再生。
Activation of Shh signaling is known to be observed following injury of the peripheral nerves such as the sciatic nerve. However, the precise role of Shh signaling during peripheral nerve regeneration is not fully understood. The inferior alveolar nerve (IAN) is most commonly injured during oral surgery. Unlike the sciatic nerve, the IAN is isolated from other craniofacial tissues, as it resides in a long bony canal within the mandible. The IAN is thus an excellent experimental model for investigating peripheral nerve regeneration. In this study, the role of Shh signaling in peripheral nerve regeneration was investigated using the mouse IAN transection model. During regeneration, Shh signaling was activated within the entire distal region of the IAN and proximal stumps. Inhibition of Shh signaling by cyclopamine application at the transection site led to abnormal axon growth in random directions, a reduced number of macrophages, and an increase in myelin debris within the distal region. Shh signaling is thus involved in peripheral nerve regeneration via the regulation of myelin degradation.