Recruitment of the human TREX complex to mRNA during splicing
Recruitment of the human TREX complex to mRNA during splicing
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DOI：
10.1101/gad.1302205
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发表时间：
2005-07-01
影响因子：
10.5
通讯作者：
Reed, R
中科院分区：
文献类型：
--
作者：
Masuda, S;Das, R;Reed, R
在酵母中，TREX复合体包含THO转录延伸复合体，其功能是将mRNA输出蛋白Sub2和Yra1直接共转录募集到新生的转录产物中。在这里，我们报告了人类Tho复合体的鉴定，结果表明，在体外，它与剪接的mRNA结合，而不与未剪接的Pre-mRNA结合。本次招聘不需要抄写。我们还发现，在体内，人类THO复合体与剪接因子在核斑点结构域中共定位。考虑到剪接在人类中是以共转录的方式发生的，我们的数据表明，人类TREX复合体与剪接的mRNA的募集并不是直接与转录耦合，而是通过剪接间接与转录耦合。
In yeast, the TREX complex contains the THO transcription elongation complex, which functions in direct co-transcriptional recruitment of the mRNA export proteins Sub2 and Yra1 to nascent transcripts. Here we report the identification of the human THO complex and show that it associates with spliced mRNA, but not with unspliced pre-mRNA in vitro. Transcription is not required for this recruitment. We also show that the human THO complex colocalizes with splicing factors in nuclear speckle domains in vivo. Considering that splicing occurs cotranscriptionally in humans, our data indicate that recruitment of the human TREX complex to spliced mRNA is not directly coupled to transcription, but is instead coupled to transcription indirectly through splicing.