Performance evaluation of endoscopic Cerenkov luminescence imaging system: in vitro and pseudotumor studies
Performance evaluation of endoscopic Cerenkov luminescence imaging system: in vitro and pseudotumor studies
复制标题
内窥镜切伦科夫发光成像系统的性能评估：体外和假瘤研究。
DOI：
10.1364/boe.5.003660
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发表时间：
2014-10-01
影响因子：
3.4
通讯作者：
Tian, Jie
中科院分区：
文献类型：
--
作者：
Cao, Xin;Chen, Xueli;Tian, Jie
By integrating the clinically used endoscope with the emerging Cerenkov luminescence imaging (CLI) technology, a new endoscopic Cerenkov luminescence imaging (ECLI) system was developed. The aim is to demonstrate the potential of translating CLI to clinical studies of gastrointestinal (GI) tract diseases. We systematically evaluated the feasibility and performance of the developed ECLI system with a series of in vitro and pseudotumor experiments. The ECLI system is comprised of an electron multiplying charge coupled device (EMCCD) camera coupled with a clinically used endoscope via an optical adapter. A 1951-USAF test board was used to measure the white-light lateral resolution, while a homemade test chart filled with (68)Ga was employed to measure the CL lateral resolution. Both in vitro and pseudotumor experiments were conducted to obtain the sensitivity of the ECLI system. The results were validated with that of CLI using EMCCD only, and the relative attenuation ratio of the ECLI system was calculated. Results showed that The white-light lateral resolution of the ECLI system was 198 µm, and the luminescent lateral resolution was better than 1 mm. Sensitivity experiments showed a theoretical sensitivity of [Formula: see text] ([Formula: see text]) and [Formula: see text] ([Formula: see text]) for the in vitro and pseudotumor studies, respectively. The relative attenuation ratio of ECLI to CLI was about 96%. The luminescent lateral resolution of the ECLI system was comparable with that of positron emission tomography (PET). The pseudotumor study illustrated the feasibility and applicability of the ECLI system in living organisms, indicating the potential for translating the CLI technology to the clinic.
