The Current Status and Future Outlook of Quantum Dot-Based Biosensors for Plant Virus Detection.
The Current Status and Future Outlook of Quantum Dot-Based Biosensors for Plant Virus Detection.
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DOI：
10.5423/ppj.rw.08.2017.0184
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发表时间：
2018-04
期刊：
影响因子：
--
通讯作者：
Lee C
中科院分区：
文献类型：
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作者：
Hong S;Lee C
酶联免疫吸附测定（ELISA）和聚合酶链反应（PCR）广泛用于植物病毒的检测，但不易进行，因此需要一种创新的、更有效的诊断方法。本文从界面元件和生物复合物的物理化学性质两个方面综述了生物传感器的特点和研究动态。特别讨论了量子点的拓扑和几何物理性质，沿着讨论了基于量子点的生物传感器及其实际应用。以量子点为基础的荧光共振能量转移（FRET）基因传感器，最广泛地应用于生物分子检测领域，和量子点为基础的纳米传感器的Rev-RRE相互作用分析作为例子。近年来，基于量子点的生物传感器已成为一类新的传感器，并有望在植物病毒检测中打开机会，但迄今为止，实际应用很少（表3）。在这篇文章中，这些案件的细节和他们的意义，未来的植物病毒检测将进行讨论。
Enzyme-linked immunosorbent assay (ELISA) and polymerase chain reaction (PCR), widely used for the detection of plant viruses, are not easily performed, resulting in a demand for an innovative and more efficient diagnostic method. This paper summarizes the characteristics and research trends of biosensors focusing on the physicochemical properties of both interface elements and bioconjugates. In particular, the topological and photophysical properties of quantum dots (QDs) are discussed, along with QD-based biosensors and their practical applications. The QD-based Fluorescence Resonance Energy Transfer (FRET) genosensor, most widely used in the biomolecule detection fields, and QD-based nanosensor for Rev-RRE interaction assay are presented as examples. In recent years, QD-based biosensors have emerged as a new class of sensor and are expected to open opportunities in plant virus detection, but as yet there have been very few practical applications (Table 3). In this article, the details of those cases and their significance for the future of plant virus detection will be discussed.