In vitro antiparasitic activity of microbial pigments and their combination with phytosynthesized metal nanoparticles.
In vitro antiparasitic activity of microbial pigments and their combination with phytosynthesized metal nanoparticles.
复制标题
微生物色素的体外抗寄生虫活性及其与植物合成金属纳米颗粒的结合。
DOI：
10.1016/j.parint.2015.05.004
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发表时间：
2015
影响因子：
1.9
通讯作者：
P. Satish
中科院分区：
文献类型：
--
作者：
S. Rahul;P. Chandrashekhar;B. Hemant;Salunke Bipinchandra;E. Mouray;P. Grellier;P. Satish
从粘质沙雷氏菌和紫罗兰色杆菌培养物中提取并纯化了生物活性色素。光谱学、红外光谱和高效液相色谱分析表明，其主要成分为芥子红素和紫罗兰素。研究了生物活性微生物色素prodigiiosin、violacein及其与植物合成的纳米银、纳米金复合对恶性疟原虫和布氏锥虫体外生长的抑制作用。对这两种寄生虫的体外抑菌效果均优于紫紫素。具体地说，微生物色素prodigiosin与金属纳米颗粒的组合显示出对两种寄生虫的ic50值显著降低（2.7至3.6倍），而对哺乳动物细胞的细胞毒性没有增加。这些数据对微生物色素类药物的设计有一定的参考价值。
Bioactive pigments were extracted and purified from cultures ofSerratia marcescensandChromobacterium violaceum. Spectroscopic, FTIR, and HPLC analyses showed prodigiosin and violacein as the principle molecules in the extract. Bioactive microbial pigments prodigiosin, violacein and their combinations with phytosynthesized silver and gold nanoparticles were studied for in vitro growth inhibition ofPlasmodium falciparumandTrypanosoma brucei gambiense. Prodigiosin was found to be more effective than violacein for inhibition of both parasites in vitro. Specifically, combinations of the microbial pigment prodigiosin with metal nanoparticles showed a significant decrease in the IC50values on both parasites (2.7 to 3.6 fold) without increase of cytotoxicity upon mammalian cells. The data may be useful for the microbial pigment based drug designing.