Accumulation of pathogenesis‐related (PR) 10/Bet v 1 protein homologues in mulberry (Morus bombycis Koidz.) tree during winter
Accumulation of pathogenesis‐related (PR) 10/Bet v 1 protein homologues in mulberry (Morus bombycis Koidz.) tree during winter
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DOI：
10.1111/j.1365-3040.2004.01216.x
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发表时间：
2004-09
影响因子：
7.3
通讯作者：
N. Ukaji;C. Kuwabara;D. Takezawa;K. Arakawa;S. Fujikawa
中科院分区：
文献类型：
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作者：
N. Ukaji;C. Kuwabara;D. Takezawa;K. Arakawa;S. Fujikawa
对从桑树皮层薄壁细胞中提取的总可溶性蛋白组分进行季节性评估，确定了与冷适应期直接相关的主要 18 kDa 蛋白。 18 kDa 蛋白质，称为 WAP18（w inter acumulated 18 kDa Proteins）从 9 月到 12 月增加，然后逐渐减少，直到 6 月。 WAP18在仲冬时检测到最高水平，这对应于桑树皮层薄壁细胞的最大耐冻能力。二维凝胶电泳证实WAP18由至少三种等电点在5.0至6.0之间的蛋白质组成。所有三种蛋白质都与抗 WAP18 抗体发生反应，从而表明它们代表了单独的亚型。此外，N端氨基酸序列分析表明，所有三种蛋白彼此具有高度的序列相似性，并且与发病机制相关(PR) - - 10/Bet v 1蛋白家族具有高度同源性。纯化的 WAP18 对冷冻不稳定的 L-乳酸脱氢酶 (LDH) 具有体外冷冻保护活性。这些结果表明WAP18可能在桑树冬季皮层薄壁细胞的耐冻机制中发挥作用。
Seasonal evaluation of total soluble protein fractions extracted from cortical parenchyma cells of mulberry ( Morus bombycis Koidz.) tree identified a predominant 18 kDa protein that was directly correlated to periods of cold acclimation. The 18 kDa protein, designated as WAP18 ( w inter a ccumulating 18 kDa p roteins) increased from September to December and then gradually decreased until June. The maximum levels of WAP18 were detected in mid-winter, which corresponds to the maximum freeze tolerance in cortical parenchyma cells of mulberry tree. Two-dimensional gel electrophoresis confirmed that WAP18 consists of at least three proteins that range between an isoelectric point of 5.0 and 6.0. All three proteins reacted with anti-WAP18 antibodies, thereby suggesting that they represent individual isoforms. Furthermore, N-terminal amino acid sequence analysis demonstrated that all three proteins contain high sequence similarity to each other and high homology to pathogenesis-related (PR) - - 10/Bet v 1 protein families. The purified WAP18 exhibited in vitro cryoprotective activity for the freeze labile L -lactate dehydrogenase (LDH) enzyme. These results suggest that WAP18 may function in the freezing tolerance mechanism of cortical parenchyma cells of mulberry tree during winter.