PROTEIN A-GOLD ELECTRON-MICROSCOPIC IMMUNOCYTOCHEMISTRY - METHODS, APPLICATIONS, AND LIMITATIONS
PROTEIN A-GOLD ELECTRON-MICROSCOPIC IMMUNOCYTOCHEMISTRY - METHODS, APPLICATIONS, AND LIMITATIONS
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DOI：
10.1002/jemt.1060010304
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发表时间：
1984-01-01
期刊：
影响因子：
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通讯作者：
BENDAYAN, M
中科院分区：
文献类型：
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作者：
BENDAYAN, M
已引入植入后蛋白A-金免疫细胞化学方法作为抗原位点超微结构定位的其他技术的替代方法。本文综述了蛋白质A-金方法的发展、理论背景和技术方法，以及为了提高结果的分辨率和在同一切片上进行双重标记而引入的不同修改。各种例子证明了这种技术的可靠性和广泛的应用。此外，一些问题，陷阱和限制，特别是这种方法的报告。
The postembedding protein A‐gold immunocytochemical approach has been introduced as an alternative to other techniques for the ultrastructural localization of antigenic sites. The present review deals with the development, the theoretical background, and technical approach of the protein A‐gold method as well as the different modifications introduced in order to enhance the resolution of the results and to perform double labelings on the same section. Various examples demonstrate the reliability and the wide range of application of this technique. In addition, some problems, pitfalls, and limitations particular to this method are reported.