New components of a system for phosphate accumulation and polyphosphate metabolism in Saccharomyces cerevisiae revealed by genomic expression analysis
New components of a system for phosphate accumulation and polyphosphate metabolism in Saccharomyces cerevisiae revealed by genomic expression analysis
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DOI：
10.1091/mbc.11.12.4309
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发表时间：
2000-12-01
影响因子：
3.3
通讯作者：
Brown, PO
中科院分区：
文献类型：
--
作者：
Ogawa, N;DeRisi, J;Brown, PO
PHO调节途径参与酵母酿酒酵母中磷酸盐（P-i）的获得。当细胞外P-i浓度低时，该途径转录诱导几种基因，包括Pho 4转录激活因子、Pho 80-Pho 85细胞周期蛋白-CDK对和Pho 81 CDK抑制剂。为了鉴定该系统调控的所有组分，采用全基因组DNA微阵列分析，鉴定了22个PHO调控基因。这些基因中有21个的启动子区至少含有一个与Pho 4识别位点相匹配的序列拷贝。这些基因中的八个，PHM 1-PHM 8，在磷酸盐代谢中没有先前定义的功能。PHM 1（YFL 004 w）、PHM 2（YPL 019 c）、PHM 3（YJL 012 c）和PHM 4（YEX 072 w）的氨基酸序列具有32-56%的同一性。phm 3和phm 4单突变体以及phm 1和phm 2双突变体均严重缺乏无机多磷酸盐（polyP）和P-i的积累。phm 5突变体的表型表明，PHM 5（YDX 452 w）是必不可少的正常catalysis的聚P在酵母液泡。两者合计，结果揭示了重要的新功能的遗传系统，在P-I收购和息肉代谢在酵母中发挥着关键作用。
The PHO regulatory pathway is involved in the acquisition of phosphate (P-i) in the yeast Saccharomyces cerevisiae. When extracellular P-i concentrations are low, several genes are transcriptionally induced by this pathway, which includes the Pho4 transcriptional activator, the Pho80-Pho85 cyclin-CDK pair, and the Pho81 CDK inhibitor. In an attempt to identify all the components regulated by this system, a whole-genome DNA microarray analysis was employed, and 22 PHO-regulated genes were identified. The promoter regions of 21 of these genes contained at least one copy of a sequence that matched the Pho4 recognition site. Eight of these genes, PHM1-PHM8, had no previously defined function in phosphate metabolism. The amino acid sequences of PHM1 (YFL004w), PHM2 (YPL019c), PHM3 (YJL012c), and PHM4 (YEX072w) are 32-56% identical. The phm3 and phm4 single mutants and the phm1 phm2 double mutant were each severely deficient in accumulation of inorganic polyphosphate (polyP) and P-i. The phenotype of the phm5 mutant suggests that PHM5 (YDX452w) is essential for normal catabolism of polyP in the yeast vacuole. Taken together, the results reveal important new features of a genetic system that plays a critical role in P-i acquisition and polyp metabolism in yeast.