Regional cerebral glucose utilization measured with [14C]glucose in brief experiments.
Regional cerebral glucose utilization measured with [14C]glucose in brief experiments.
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在简短的实验中用[14C]葡萄糖测量局部脑葡萄糖利用率。
DOI：
10.1152/ajpcell.1983.245.5.c428
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发表时间：
1983
期刊：
影响因子：
--
通讯作者：
Hawkins,RA
中科院分区：
文献类型：
--
作者：
Lu,DM;Davis,DW;Mans,AM;Hawkins,RA
开发了一种使用放射自显影和[2- 14C]葡萄糖在非常短的时间内测量局部脑葡萄糖利用率（rCMRGlc）的方法。它包含比原型更全面的数学处理，并允许更大的实验灵活性。当前模型考虑了血浆和大脑之间的葡萄糖交换，作为血浆葡萄糖浓度和 rCMRGlc 的函数。 14C 作为 14CO2 的损失虽然相对较小，但也进行了计算并对结果进行了相应修正。选择完全可代谢的放射性标记葡萄糖作为示踪剂，因为它最接近天然底物。葡萄糖的磷酸化速度比 2-脱氧葡萄糖等类似物更快，中间代谢物中积累的 14C 很快就占据了脑组织中的大部分放射性。此外，不需要像使用类似物时那样调整速率的特殊常数。当该技术用于在短至 5 分钟的实验中确定 rCMRGlc 时，实现了出色的解剖分辨率。通过采用这种方法，可以在比以前更多样化的条件下研究更多的短暂现象。
A method was developed to measure regional cerebral glucose utilization (rCMRGlc) in very short times using autoradiography and [2- 14C]glucose. It includes a more comprehensive mathematical treatment than prototypes and allows greater experimental flexibility. The current model takes into account the exchange of glucose between plasma and brain as a function of the plasma glucose concentration and rCMRGlc. The loss of 14C as 14CO2, although relatively small, is also calculated and the results corrected accordingly. Fully metabolizable radiolabeled glucose was chosen as the tracer, because it most closely resembles the natural substrate. Glucose is more rapidly phosphorylated than analogues such as 2-deoxyglucose and the 14C accumulated within intermediary metabolites soon account for most of the radioactivity in cerebral tissue. Furthermore, no special constant is necessary to adjust the rates as is the case when analogues are used. Excellent anatomic resolution was achieved when the technique was used to determine rCMRGlc in experiments as short as 5 min. By taking this approach, more short-lived phenomena may be studied under more diverse conditions than was previously possible.