RNA Polymerase III?Dependent <i>BoNR8</i> and <i>AtR8</i> lncRNAs Contribute to Hypocotyl Elongation in Response to Light and Abscisic Acid
RNA Polymerase III?Dependent <i>BoNR8</i> and <i>AtR8</i> lncRNAs Contribute to Hypocotyl Elongation in Response to Light and Abscisic Acid
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RNA 聚合酶 III？依赖 <i>BoNR8</i> 和 <i>AtR8</i> lncRNA 响应光和脱落酸促进下胚轴伸长
DOI：
10.1093/pcp/pcad025
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发表时间：
2023
影响因子：
4.9
通讯作者：
Wu Juan
中科院分区：
文献类型：
--
作者：
Zhang Nan;Xu Kai;Liu Shengyi;Yan Rong;Liu Ziguang;Wu Ying;Peng Yifang;Zhang Xiaoxu;Yukawa Yasushi;Wu Juan
下胚轴伸长受光抑制，黑暗促进。植物激素脱落酸（阿坝）也抑制下胚轴伸长。然而，光和阿坝信号对下胚轴伸长的综合作用的分子机制的细节仍不清楚。长链非编码RNA（lncRNA; >200 nt）不编码蛋白质，但在生物体中发挥许多生理作用。到目前为止，只有少数与下胚轴伸长相关的lncRNA被报道。lncRNABoNR 8（272 nt）和AtR 8（259 nt）均由RNA聚合酶III转录，是分别在甘蓝和拟南芥中大量存在的同源lncRNA。这些lncRNA共享77%的序列同一性，其预测的RNA二级结构是相似的，在两个序列中的非保守核苷酸主要位于茎环区的二级结构。先前的研究表明BoNR 8沿着阿坝调节种子萌发，AtR 8可能参与拟南芥的先天免疫功能。我们的研究结果表明，BoNR 8和AtR 8的表达水平受到光和阿坝的不同影响，并且在拟南芥中，BoNR 8和AtR 8的过表达（OX）依赖于光和阿坝调节下胚轴的伸长。在AtR 8-OX和BoNR 8-OX中，光相关基因PHYB、COP 1、HY 5和PIF 4以及ABA相关基因ABI 3和ABI 5的表达水平发生了变化，而在ABI 3缺陷型突变体中，阿坝的加入显著提高了下胚轴在黑暗条件下的伸长。这些结果表明，BoNR 8和AtR 8与ABI 3和关键下游光信号基因一起调节下胚轴的伸长。
Hypocotyl elongation is inhibited by light and promoted by darkness. The plant hormone abscisic acid (ABA) also inhibits hypocotyl elongation. However, details of the molecular mechanism that regulates the integrated effects of light and ABA signaling on hypocotyl elongation remain unclear. Long non-coding RNAs (lncRNAs; >200 nt) do not encode proteins but play many physiological roles in organisms. Until now, only a few lncRNAs related to hypocotyl elongation have been reported. The lncRNAsBoNR8(272 nt) andAtR8(259 nt), both of which are transcribed by RNA polymerase III, are homologous lncRNAs that are abundantly present in cabbage andArabidopsis, respectively. These lncRNAs shared 77% sequence identity, and their predicted RNA secondary structures were similar; the non-conserved nucleotides in both sequences were positioned mainly in the stem–loop regions of the secondary structures. A previous study showed thatBoNR8regulated seed germination along with ABA and thatAtR8may be involved in innate immune function inArabidopsis. Our results show that the expression levels ofBoNR8andAtR8were differentially affected by light and ABA and that overexpression (OX) of bothBoNR8andAtR8in Arabidopsis regulated hypocotyl elongation depending on light and ABA.. The expression levels of light-related genesPHYB, COP1, HY5andPIF4and ABA-related genesABI3andABI5were altered in theAtR8-OX andBoNR8-OX lines, and, in anABI3-defective mutant, hypocotyl elongation was greatly increased under dark condition with the addition of ABA. These results indicate thatBoNR8andAtR8regulate hypocotyl elongation together withABI3and key downstream light signaling genes.