Highly sensitive fluorescence-based mercury(II) DNA sensor enhanced by silver(I) activation
Highly sensitive fluorescence-based mercury(II) DNA sensor enhanced by silver(I) activation
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DOI：
10.1016/j.measen.2022.100368
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发表时间：
2022-03
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通讯作者：
N. Idros;Katherine M. Stott;Jasmina Allen;V. S. Kamboj;W. Corns;H. Verde-Luján;L. Valladares;Carlos Villanueva;J. Jhoncon;Daping Chu;C. Barnes
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作者：
N. Idros;Katherine M. Stott;Jasmina Allen;V. S. Kamboj;W. Corns;H. Verde-Luján;L. Valladares;Carlos Villanueva;J. Jhoncon;Daping Chu;C. Barnes
汞接触对人类健康和环境构成严重风险。基于对痕量汞的监测需求，我们报道了一种高灵敏度的汞DNA传感器，该传感器通过银（I）活化、汞（II）特异性寡核苷酸（MSO）分子构型转换和汞（II）调节的FRET增强。用银（I）激活MSO链，错配的胞嘧啶-银（I）-胞嘧啶桥诱导单独的MSO链响应于汞（II）而容易地折叠，导致增强的荧光信号。通过2D 1H-1H NOESY和TOCSY NMR光谱和260 nm吸光度研究了结构开关。信号随着汞（II）浓度从100 pM增加到0.1 mM而减小。该方法相对于其他与环境相关的金属具有突出的汞（II）选择性。此外，该方法用于检测汞（II）在加标池塘沃茨与97.9-100.6%的回收率。这种简单可行的格式对于开发一种具有成本效益和有用的环境监测工具具有很大的潜力。
Mercury exposure constitutes an acute risk to human health and the environment. Driven by the requirement to monitor trace-level mercury, we report a highly sensitive mercury(II) DNA sensor enhanced by silver(I) activation, followed by mercury(II)-specific oligonucleotides (MSO) molecular configuration switch and mercury(II)-modulated FRET. Activating the MSO strands with silver(I), mismatched cytosine–silver(I)–cytosine bridges induce individual MSO strands to fold readily in response to mercury(II) resulting in enhanced fluorescence signal. The structural switches were studied by 2D1H–1H NOESY and TOCSY NMR spectroscopy, and 260 nm absorbance. The signal decreases with increasing mercury(II) concentration from 100 pM to 0.1 mM. The approach affords outstanding mercury(II) selectivity over other environmentally associated metals. Furthermore, the methodology was deployed for detection of mercury(II) in spiked pond waters with 97.9–100.6% recovery. The simple and feasible format has great potential for developing a cost effective and useful tool for environmental monitoring.