A multifunctional label-free electrochemical impedance biosensor for Hg2+, adenosine triphosphate and thrombin
A multifunctional label-free electrochemical impedance biosensor for Hg2+, adenosine triphosphate and thrombin
复制标题
一种多功能无标记电化学阻抗生物传感器，可检测汞、三磷酸腺苷和凝血酶
DOI：
10.1016/j.talanta.2014.10.039
复制
发表时间：
2015-01-15
期刊：
影响因子：
6.1
通讯作者：
Chen, Zhong-Ning
中科院分区：
文献类型：
--
作者：
Chen, Lifen;Chen, Zhong-Ning
基于核酸亚基与不同靶分子相互作用时，修饰电极的电化学阻抗谱（EIS）的变化，建立了一种检测Hg 2+、三磷酸腺苷和凝血酶的多功能无标记生物传感器。该修饰电极由三个相互作用部分组成，分别是T-T错配的DNA与Hg ~（2+）形成T-Hg ~（2+）-T复合物，断裂DNA芯片与ATP，DNA domin与凝血酶形成G-四链体。当DNA与凝血酶或ATP相互作用时，已检测到增加的电荷转移电阻（R-ct）。然而，获得了对Hg 2+的降低的R-ct。R-ct差值（Δ R-ct）与不同靶点的浓度有关，Hg 2+、ATP和凝血酶可被选择性地检测，检测限分别为0.03、0.25和0.20 nmol L-1。分别检测同一样品中存在的三种分析物。分别用ATP适配体、富G DNA链和EDTA掩蔽ATP、Hg ~（2+）和凝血酶。(C)2014爱思唯尔有限公司版权所有。
A multifunctional label-free biosensor for the detection of Hg2+, adenosine triphosphate and thrombin has been developed based on the changing of the electrochemical impedance spectroscopy (EIS) from the modified electrodes when nucleic acid subunits interacting with different targets. The modified electrode consists of three interaction sections, including DNA with T-T mismatch recognizing Hg2+ to form T-Hg2+-T complex, split DNA chip against ATP, and DNA domin against thrombin to form G-quadruplex. Upon DNA interaction with thrombin or ATP, an increased charge transfer resistance (R-ct) had been detected. However, a decreased R-ct against Hg2+ was obtained. The R-ct difference (Delta R-ct) has relationship with the concentration of the different targets, Hg2+, ATP and thrombin can be selectively detected with the detection limit of 0.03, 0.25, and 0.20 nmol L-1, respectively. To separately detect the three analytes existing in the same sample. ATP aptamer, G-rich DNA strands and EDTA were applied to mask ATP, Hg2+ or thrombin separately. (C) 2014 Elsevier B.V. All rights reserved.