Characterization of Pepper yellow leaf curl virus, a tentative new Polerovirus species causing a yellowing disease of pepper
Characterization of Pepper yellow leaf curl virus, a tentative new Polerovirus species causing a yellowing disease of pepper
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DOI：
10.1007/s12600-010-0120-x
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发表时间：
2010-11-01
期刊：
影响因子：
1.4
通讯作者：
Antignus, Yehezkel
中科院分区：
文献类型：
--
作者：
Dombrovsky, Aviv;Glanz, Eyal;Antignus, Yehezkel
辣椒（Capsicum annuum）是世界上重要的农作物。在以色列，大约有2，500公顷土地全年种植，供应当地和出口市场。在此，我们报告了一种病毒病原体的鉴定，该病原体引起辣椒作物的一种新的毁灭性疾病。该病害的症状包括茎节间缩短、脉间变黄、叶片向上卷曲，并伴有果实变色和体积缩小。从感染的植物中纯化病毒产生直径为25 nm的等轴颗粒。病原暂定名为辣椒黄化曲叶病毒（PYLCV）。该病毒在DAS ELISA中与瓜蚜传黄化病毒和马铃薯卷叶病毒（Polerovirus属成员）的抗血清发生交叉反应。从克隆的病毒外壳蛋白和运动蛋白基因获得的部分核苷酸序列表明，在氨基酸水平上与Polerovirus属的另一个成员烟草脉扭曲病毒（TVDV）的92%的一致性。然而，PYLCV的宿主范围与TVDV描述的宿主范围显著不同。根据我们的研究结果，PYLCV的分类地位进行了讨论。
Pepper (Capsicum annuum) is an important crop worldwide. In Israel, approximately 2,500 ha are grown all year round for the local and export markets. Herein, we report the identification of a viral pathogen causing a new devastating disease in pepper crops. The disease syndrome includes shortening of stem internodes, interveinal yellowing, and upward rolling of the leaf blade, accompanied by fruit discoloration and size reduction. Virus purification from infected plants yielded isometric particles, 25 nm in diameter. The causal agent of the disease was tentatively named Pepper yellow leaf curl virus (PYLCV). The virus cross-reacted in DAS ELISA with antisera against Cucurbit aphid-borne yellows virus and Potato leafroll virus, members of the Polerovirus genus. The partial nucleotide sequence obtained from the cloned viral coat protein and movement protein genes indicated 92% identity at the amino acid level with Tobacco vein distortion virus (TVDV), another member of the Polerovirus genus. However, the host range of PYLCV is significantly different from the host range described for TVDV. Based on our findings, the taxonomic status of PYLCV is discussed.