Magnetic resonance imaging accurately estimates LV mass in a transgenic mouse model of cardiac hypertrophy
Magnetic resonance imaging accurately estimates LV mass in a transgenic mouse model of cardiac hypertrophy
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DOI：
10.1152/ajpheart.1998.274.2.h679
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发表时间：
1998-02-01
影响因子：
4.8
通讯作者：
Shohet, RV
中科院分区：
文献类型：
--
作者：
Franco, F;Dubois, SK;Shohet, RV
具有功能失调的关酰环化酶a基因（GCA -/-）的转基因小鼠不能转导心房自然肽的信号，从而产生高血压和心脏肥厚，并以野生型兄弟姐妹为对照，采用磁共振成像（MRI）来评估这些动物的心脏肥厚。麻醉小鼠在1.5 t下采用门控多层、多相电影MRI进行研究，利用辛普森规则从短轴图像估计左心室（LV）的质量和体积。MRI评估的左室质量与尸检的相关性很好，左室尸检= 1.04 x LVMRI + 4.69 mg （r(2) = 0.95）。通过MRI，与等基因对照组相比，GCA -/-左室质量显著不同[GCA -/-， 226 +/- 43 mg （n = 14）与对照组，156 +/- 14 mg (n = 10)；P < 0.0001]。两组左室容积和射血分数差异无统计学意义。MRI为小鼠心脏表型的无创评估提供了一种准确的手段，并且可能在跟踪基因修饰的影响方面有用。
Transgenic mice with a dysfunctional guanylyl cyclase A gene (GCA -/-) are unable to transduce the signals from atrial naturetic peptide and develop hypertension and cardiac hypertrophy Magnetic resonance imaging (MRI) was performed to assess cardiac hypertrophy in these animals, using wild-type siblings as controls. Anesthetized mice were studied by gated multislice, multiphase cine MRI at 1.5 T. Simpson's rule was used to estimate left ventricle (LV) mass and volumes from short-axis images. Correlation between LV mass evaluated by MRI and at necropsy was excellent, with LVnecropsy = 1.04 x LVMRI + 4.69 mg (r(2) = 0.95). By MRI, GCA -/- LV mass was significantly different when compared with isogenic controls [GCA -/-, 226 +/- 43 mg (n = 14) vs. controls, 156 +/- 14 mg (n = 10); P < 0.0001]. LV volumes and ejection fraction in the two groups were not significantly different. MRI provides an accurate means for the noninvasive assessment of murine cardiac phenotype and may be useful in following the effects of genetic modification.