Enhancing Osteogenic Differentiation of MC3T3-E1 Cells by Immobilizing Inorganic Polyphosphate onto Hyaluronic Acid Hydrogel
Enhancing Osteogenic Differentiation of MC3T3-E1 Cells by Immobilizing Inorganic Polyphosphate onto Hyaluronic Acid Hydrogel
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DOI：
10.1021/bm501356c
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发表时间：
2015-01-01
影响因子：
6.2
通讯作者：
Furukawa, Katsuko S.
中科院分区：
文献类型：
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作者：
Wu, Andy T. H.;Aoki, Teruo;Furukawa, Katsuko S.
在组织工程中，精确控制微环境中的信号对于刺激细胞进行生物活性如增殖、分化和基质产生至关重要。然而，目前的方法在提供非消耗性线索方面效率低下。在这项研究中，我们开发了一种新型的生物活性水凝胶（HAX-PolyP），能够通过将无机聚磷酸盐链缀合到透明质酸大分子单体上来增强组织生长。固定化的聚磷酸盐为嵌入的鼠成骨细胞前体细胞提供恒定的骨传导刺激，导致成骨标志物基因的上调和ALP活性水平的提高。骨传导活性显着更高时，与自由浮动的聚磷酸盐刺激。即使在非常低的浓度下，多磷酸盐固定在支架上也可以产生足够的信号，从而产生更有效的骨传导。这些结果证明了我们的新型材料作为可注射生物活性支架的潜力，其可以在临床上用于开发骨移植物和骨再生应用。
In tissue engineering, precise control of cues in the microenvironment is essential to stimulate cells to undergo bioactivities such as proliferation, differentiation, and matrix production. However, current approaches are inefficient in providing nondepleting cues. In this study, we have developed a novel bioactive hydrogel (HAX-PolyP) capable of enhancing tissue growth by conjugating inorganic polyphosphate chains onto hyaluronic acid macromers. The immobilized polyphosphates provided constant osteoconductive stimulation to the embedded murine osteoblast precursor cells, resulting in up-regulation of osteogenic marker genes and enhanced levels of ALP activity. The osteoconductive activity was significantly higher when compared to those stimulated with free-floating polyphosphates. Even at very low concentrations, immobilization of polyphosphates onto the scaffold allowed sufficient signaling leading to more effective osteoconduction. These results demonstrate the potential of our novel material as an injectable bioactive scaffold, which can be clinically useful for developing bone grafts and bone regeneration applications.