RATE OF CHOLESTERYL ESTER TRANSFER BETWEEN HIGH AND LOW-DENSITY LIPOPROTEINS IN HUMAN-SERUM AND A CASE WITH DECREASED TRANSFER RATE IN ASSOCIATION WITH HYPERALPHALIPOPROTEINEMIA
RATE OF CHOLESTERYL ESTER TRANSFER BETWEEN HIGH AND LOW-DENSITY LIPOPROTEINS IN HUMAN-SERUM AND A CASE WITH DECREASED TRANSFER RATE IN ASSOCIATION WITH HYPERALPHALIPOPROTEINEMIA
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DOI：
10.1093/oxfordjournals.jbchem.a135418
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发表时间：
1985-01-01
影响因子：
2.7
通讯作者：
YAMAMOTO, A
中科院分区：
文献类型：
--
作者：
KURASAWA, T;YOKOYAMA, S;YAMAMOTO, A
在不存在卵磷脂：胆固醇酰基转移酶（LCAT）和极低密度脂蛋白的情况下，在人血清中测定了内在高密度脂蛋白和低密度脂蛋白（HDL和LDL）之间胆固醇酯转移的速率。根据两个脂蛋白胆固醇酯池之间的平衡转移模型计算速率。平均转移率为213 .+-. 95 nmol/h/ml（平均值±.（S. E.）所有20名正常受试者和13名高脂蛋白血症受试者。个体血清中的转移率比胆固醇酯化率高出数倍，表明LCAT对HDL产生的胆固醇酯迅速分布在各种脂蛋白亚类中。血清中胆固醇酯转移的速率与固有HDL和LDL浓度的乘积成显著比例，这是这些脂蛋白之间碰撞频率的可能指标，用于生理脂蛋白浓度的范围。在分析的受试者中，有一名患者患有显著的高脂蛋白血症并伴有高胆固醇血症，其胆固醇酯转移率与LDL和HDL浓度相关性非常低。对患者的脂蛋白脂质成分分析也支持血液中脂蛋白之间中性脂质转移活性受损的可能性。
The rate of cholesteryl ester transfer between intrinsic high and low density lipoproteins (HDL and LDL) was measured in human serum in the absence of lecithin: cholesterol acyltransferase (LCAT) and very low density lipoproteins. The rate was calculated according to the equilibrium transfer model between the two lipoprotein cholesteryl ester pools. The average rate of the transfer was 213 .+-. 95 nmol/h/ml (mean .+-. S.E.) for all 20 normal and 13 hyperlipoproteinemic subjects. The transfer rate in individual serum was higher by several times than the cholesterol esterification rate, showing that cholesteryl ester generated by LCAT on HDL is promptly distributed among various lipoprotein subclasses. The rate of cholesteryl ester transfer in the serum was significantly proportional to the product of intrinsic HDL and LDL concentrations, a possible indicator of the frequency of collision between these lipoproteins, for the range of physiological lipoprotein concentrations. Among the subjects analyzed was a patient with remarkable hyperalphalipoproteinemia accompanied by hypertriglyceridemia, who showed a very low rate of cholesteryl ester transfer in relation to his LDL and HDL concentrations. Compositional analysis of lipoprotein lipids of the patients also supported the possibility of impaired neutral lipids transfer activity among lipoproteins in the blood.