Photoisomerization of retinal at 13-ene is important for phototaxis of Chlamydomonas reinhardtii: simultaneous measurements of phototactic and photophobic responses.
Photoisomerization of retinal at 13-ene is important for phototaxis of Chlamydomonas reinhardtii: simultaneous measurements of phototactic and photophobic responses.
复制标题
13-烯视网膜的光异构化对于莱茵衣藻的趋光性很重要：同时测量趋光和避光反应。
DOI：
10.1016/0006-291x(91)91031-7
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发表时间：
1991
影响因子：
3.1
通讯作者：
Nakanishi,K
中科院分区：
文献类型：
--
作者：
Takahashi,T;Yoshihara,K;Watanabe,M;Kubota,M;Johnson,R;Derguini,F;Nakanishi,K
建立了一种实时自动化的方法，用于同时测量鞭毛微生物的趋光性（趋光性）和步升疏水反应。添加全transretinal以剂量依赖性方式恢复了类胡萝卜素缺陷型莱氏衣原体突变株的两种光反应。趋光性取向与光强和视网膜浓度呈双相性。全transretinal比11-cisretinal更有效地再生两种光行为反应。类似物具有锁定的11-顺式构型和侧链中的苯环也诱导光响应，尽管浓度比全transretinal高两个数量级以上。根据本检测方法，在与视网膜类似物孵育的细胞中几乎检测不到反应，其中13-烯被锁定在其反式顺式构型中。结果表明，13 - 14双键的异构化是重要的光行为信号转导和一个单一的视网膜依赖性光感受器控制趋光和疏水反应。
A real-time automated method was developed for simultaneous measurements of phototactic orientation (phototaxis) and step-up photophobic response of flagellated microorganisms. Addition of all-transretinal restored both photoresponses in a carotenoid-deficient mutant strain ofChlamydomonas reinhardtiiin a dose-dependent manner. The phototactic orientation was biphasic with respect to both the light intensity and the concentration of retinal. All-transretinal was more effective than 11-cisretinal to regenerate both photobehavioral responses. Analogs having locked 11-cisconfigurations and a phenyl ring in the side chain also induced photoresponses, although at concentrations more than two orders of magnitude higher than all-transretinal. According to the present assay method, the responses were hardly detectable in cells incubated with retinal analogs in which the 13-ene was locked in either itstransorcisconfiguration. The results strongly suggest that the isomerization of the 13–14 double bond is important for photobehavioral signal transduction and that a single retinal-dependent photoreceptor controls both phototactic and photophobic responses.