The immobilization antigens of Tetrahymena thermophila are glycoproteins.
The immobilization antigens of Tetrahymena thermophila are glycoproteins.
复制标题
嗜热四膜虫的固定化抗原是糖蛋白。
DOI：
10.1111/j.1550-7408.1992.tb04840.x
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发表时间：
1992
期刊：
影响因子：
--
通讯作者：
Doerder,FP
中科院分区：
文献类型：
--
作者：
Ron,A;Williams,NE;Doerder,FP
在嗜热四膜虫中由SerH基因座控制的四种固定抗原已经被分离和部分表征（Doerder，F. P. & Berkowitz，M. S. 1986.嗜热四膜虫H固定化抗原的纯化和部分特性J. Protozool.，33：204-208）。我们在这里显示，在用35 S-甲硫氨酸、14 C-甘露糖、14 C-葡糖胺和N-乙酰基-d-[1 - 3 H]葡糖胺标记后，使用免疫沉淀和电泳，这些蛋白质是糖基化的。我们认为四膜虫的固定抗原可能是通过磷脂酰肌醇聚糖锚定在膜表面的。
The four immobilization antigens controlled by theSerHlocus inTetrahymena thermophilahave been isolated and partially characterized (Doerder, F. P. & Berkowitz, M. S. 1986. Purification and partial characterization of the H immobilization antigens ofTetrahymena thermophila. J. Protozool.,33:204–208). We show here, using immunoprecipitation and electrophoresis after labeling with35S‐methionine,14C‐mannose,14C‐glucosamine, and N‐Acetyl‐d‐[1‐3H]glucosamine, that these proteins are glycosylated. We suggest the immobilization antigens inTetrahymenamay be anchored to the surface membrane by phosphatidylinositol glycans.