Effect of substance P injection into the nucleus tractus solitarius of rats on cricothyroid and thyroarytenoid motor activity and cardiovascular and respiratory systems.
Effect of substance P injection into the nucleus tractus solitarius of rats on cricothyroid and thyroarytenoid motor activity and cardiovascular and respiratory systems.
复制标题
大鼠孤束核注射 P 物质对环甲腺和甲杓肌运动活动以及心血管和呼吸系统的影响。
DOI：
10.1177/000348940211101003
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发表时间：
2002
期刊：
影响因子：
--
通讯作者：
Talman,WilliamT
中科院分区：
文献类型：
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作者：
Bauman,NancyM;Wang,DeQiang;Luschei,ErichS;Talman,WilliamT
鉴定介导喉内收肌和/或张肌活动的中枢神经递质可能有助于管理喉痉挛或明显危及生命事件中发生的病理性喉内收。推测的递质P物质（SP）被发现在孤束核（NTS），喉传入终止。因此，我们研究了SP注入大鼠NTS的喉，心血管和呼吸的影响。在30只麻醉大鼠的孤束核（NTS）、运动背核（DMN）或薄束核（GR）内，分别注射20 nL SP（15 μmol）或对照液。比较膈肌、环甲肌（CT）和甲杓肌（TA）肌电图（EMG）以及血压（BP）的变化。对注射部位进行组织学验证。将SP注入NTS改变了所有动物的CT和/或TA EMG活动。变化范围从完全抑制到阶段性增加，再到强直性增加。9只动物中有8只在DMN（4/5）或GR（4/4）注射SP后喉内收肌EMG活动无变化，但1只动物在DMN注射SP后表现出短暂的CT和TA EMG活动抑制。将SP注射到NTS中诱导所有动物的中枢性呼吸暂停和血压显著降低。NTS注射后呼吸暂停的持续时间往往长于DMN或GR注射后（分别为p <0.10和p <0.05）。我们的结论是，立体定向注射假定的神经递质在大鼠可能完成喉运动活动的影响进行鉴定。将SP直接应用于NTS中一致地引起CT和/或TA EMG活动的变化，范围从抑制到兴奋。该模型可用于评价中枢反射活动的药理学靶点，以管理危及生命的喉反射活动。
Identification of central neurotransmitters that mediate laryngeal adductor and/or tensor activity may prove useful in managing pathological laryngeal adduction as occurs in laryngospasm or apparent life-threatening events. The putative transmitter substance P (SP) is found in the nucleus tractus solitarius (NTS), in which laryngeal afferents terminate. Therefore, we studied the laryngeal, cardiovascular, and respiratory effects of SP injected into the NTS of rats. We completed bilateral stereotactic injections of 20 nL of SP (15 μmol) or control solution into the region of the NTS, the dorsal motor nucleus (DMN), or the nucleus gracilis (GR) in 30 anesthetized rats. Changes in diaphragm, cricothyroid (CT), and thyroarytenoid (TA) electromyography (EMG), as well as blood pressure (BP), were compared. The injection sites were verified histologically. Injection of SP into the NTS altered CT and/or TA EMG activity in all animals. The change ranged from complete inhibition, to a phasic increase, to a tonic increase. No change in laryngeal adductor EMG activity was seen in 8 of 9 animals after SP injections into the DMN (4/5) or GR (4/4), but 1 animal demonstrated brief inhibition of CT and TA EMG activity after SP injection into the DMN. Injection of SP into the NTS induced central apnea and a significant decrease in BP in all animals. The duration of apnea tended to be longer after NTS injections than after DMN or GR injections (p < .10 and p < .05, respectively). We conclude that stereotactic injections of putative neurotransmitters in rats may be accomplished to identify effects on laryngeal motor activity. Direct application of SP into the NTS consistently elicits a change in CT and/or TA EMG activity, ranging from inhibition to excitation. This model may prove useful in evaluating pharmacological targets of central reflex activity to manage life-threatening laryngeal reflex activity.