Calcium- and cyclic AMP-dependent chloride secretion in human colonic epithelia.
Calcium- and cyclic AMP-dependent chloride secretion in human colonic epithelia.
复制标题
人结肠上皮细胞中钙和环 AMP 依赖性氯化物分泌。
DOI：
10.1111/j.1476-5381.1987.tb11243.x
复制
发表时间：
1987
影响因子：
7.3
通讯作者：
MacVinish,LJ
中科院分区：
文献类型：
--
作者：
Cuthbert,AW;Egléme,C;Greenwood,H;Hickman,ME;Kirkland,SC;MacVinish,LJ
1在胶原蛋白包被的Millipore过滤器上培养了三种稳定的上皮细胞系（HCA-7、HCA-7-Col 1和HCA-7-Col 3），这些细胞系均来源于相同的人腺癌。这些上皮细胞单层已被用来记录短路电流（SCC）响应于各种促分泌素。类似的单层，但在塑料皿上生长，用于测量组织环AMP。2赖氨酰缓激肽，应用于单层的任何一侧，增加了HCA-7细胞中的SCC，但对其他两个线几乎没有影响。这些反应显示出快速脱敏，这可以通过冷却到4°C来防止。吡罗昔康（一种环加氧酶抑制剂）对激肽的反应没有显著减弱。3其他促分泌素、血管活性肠多肽（VIP）和卡巴胆碱也增加了单层SCC。HCA-7-Col 1单层中对VIP的反应最大，而HCA-7-Col 3中几乎不存在反应。使用卡巴胆碱观察到类似的特征，除了HCA-7和HCA-7-Col 1单层的响应更相等。除了一个例外，对VIP和卡巴胆碱的反应显示出单侧性，仅从基底外侧起作用。4促分泌素的作用被基底外侧应用的袢利尿剂piretanide抑制。据推测，SCC反应代表产电氯化物分泌。5毛喉素治疗增加了HCA-7和HCA-7-Col 1单层的SCC，对HCA-7-Col 3几乎没有影响。然而，环AMP水平在HCA-7-Col 3中升高最多，在HCA-7-Col 1单层中升高最少，当应用于HCA-7和HCA-7-Col 3单层时，6A 23187增加了SCC，对HCA-7-Col 1.7的影响很小。三种人类细胞系的差异反应为发现参与氯化物的实体的功能责任提供了独特的机会。分泌过程HCA-7-Col 3细胞响应于毛喉素产生高水平的环AMP，但未能显示实质性的氯化物分泌反应，可能是某些疾病的有用模型。
1Three stable epithelial cell lines (HCA‐7, HCA‐7‐Col 1 and HCA‐7‐Col 3) all derived from the same human adenocarcinoma have been cultured on collagen‐coated Millipore filters. These epithelial monolayers have been used to record short circuit current (SCC) in response to a variety of secretagogues. Similar monolayers, but grown on plastic dishes, were used for measurements of tissue cyclic AMP.2Lysylbradykinin, applied to either side of the monolayers, increased SCC in HCA‐7 cells but had little effect on the other two lines. The responses showed rapid desensitization, which could be prevented by cooling to 4°C. Responses to kinin were not significantly attenuated by piroxicam, an inhibitor of cyclo‐oxygenase.3Other secretagogues, vasoactive intestinal polypeptide (VIP) and carbachol also increased SCC in monolayers. The responses to VIP were greatest in HCA‐7‐Col 1 monolayers while responses were virtually absent in HCA‐7‐Col 3. A similar profile was seen with carbachol except that responses of HCA‐7 and HCA‐7‐Col 1 monolayers were more equal. With one exception the responses to VIP and carbachol showed sidedness, acting only from the basolateral side.4The effects of the secretagogues were inhibited by piretanide, a loop diuretic, applied basolaterally. It is presumed that SCC responses represent electrogenic chloride secretion.5Treatment with forskolin increased SCC in HCA‐7 and HCA‐7‐Col 1 monolayers with little effect in HCA‐7‐Col 3. Nevertheless cyclic AMP levels were elevated most in HCA‐7‐Col 3 and least in HCA‐7‐Col 1 monolayers, in reciprocal relationship to the functional response.6A23187 increased SCC when applied to HCA‐7 and HCA‐7‐Col 3 monolayers with little effect on HCA‐7‐Col 1.7The differential responses of the three human cell lines provide unique opportunities to discover the functional responsibilities of entities involved in the chloride secretory process. HCA‐7‐Col 3 cells which generate high levels of cyclic AMP in response to forskolin but which fail to show a substantial chloride secretory response may be a useful model of some disease conditions.