Spike timing-dependent plasticity alters electrosensory neuron synaptic strength in vitro but does not consistently predict changes in sensory tuning in vivo
Spike timing-dependent plasticity alters electrosensory neuron synaptic strength in vitro but does not consistently predict changes in sensory tuning in vivo
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DOI：
10.1152/jn.00498.2022
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发表时间：
2023-05-01
影响因子：
2.5
通讯作者：
Carlson，Bruce A.
中科院分区：
文献类型：
--
作者：
Lube，Adalee J.;Ma，Xiaofeng;Carlson，Bruce A.
当感觉环境不断变化时，感觉系统如何优化对行为相关刺激的检测？我们解决了尖峰时间依赖性可塑性（STDP）在驱动的感觉通路中的突触强度的变化，以及这些突触强度的变化是否可以改变感觉调谐的作用。精确控制体内突触活动的时间模式并以行为相关的方式在体外复制这些模式是具有挑战性的。这使得STDP诱导的突触生理学变化与感觉系统的可塑性之间难以建立联系。利用能产生用于电定位和通信的电器官放电的长颌鱼种Brevimyrus niger和Brienomyrus brachyistius，我们可以精确地控制体内突触输入的时间，并在体外复制这些相同的突触输入时间模式。在电通信通路中的中央感觉神经元中，使用体外全细胞细胞内记录，我们将突触前输入与突触后尖峰在不同的延迟配对。在清醒的行为鱼中使用全细胞细胞内记录，我们使用相同的延迟将感觉刺激与突触后尖峰配对。我们发现，Hebbian STDP可预见地改变感觉调谐在体外，是由NMDA受体介导的。然而，在体内感觉刺激诱导的突触反应的变化并没有坚持预测的方向在体外观察到的STDP。进一步的分析表明，这种差异受到多突触活动的影响，包括抑制性中间神经元。我们的研究结果表明，STDP规则在确定的突触操作可能不会驱动可预测的变化，在电路水平的感觉反应。新&值得注意的是，我们复制了行为相关的时间模式的突触活动在体外，并使用相同的模式在体内感觉刺激。有一个Hebbian尖峰时间依赖可塑性（STDP）模式在体外，但在体内的感觉反应并没有根据STDP的预测转移。分析表明，这种差异受到多突触活动差异的影响，包括抑制性中间神经元。这些结果表明，体外突触的STDP规则不一定适用于体内回路。https://jneurophysiol.podbean.com/e/jnp-micro-podcasts-four-questions-adalee-j-lube-and-bruce-a-carlson/
How do sensory systems optimize detection of behaviorally relevant stimuli when the sensory environment is constantly changing? We addressed the role of spike timing-dependent plasticity (STDP) in driving changes in synaptic strength in a sensory pathway and whether those changes in synaptic strength could alter sensory tuning. It is challenging to precisely control temporal patterns of synaptic activity in vivo and replicate those patterns in vitro in behaviorally relevant ways. This makes it difficult to make connections between STDP-induced changes in synaptic physiology and plasticity in sensory systems. Using the mormyrid speciesBrevimyrus nigerandBrienomyrus brachyistius, which produce electric organ discharges for electrolocation and communication, we can precisely control the timing of synaptic input in vivo and replicate these same temporal patterns of synaptic input in vitro. In central electrosensory neurons in the electric communication pathway, using whole cell intracellular recordings in vitro, we paired presynaptic input with postsynaptic spiking at different delays. Using whole cell intracellular recordings in awake, behaving fish, we paired sensory stimulation with postsynaptic spiking using the same delays. We found that Hebbian STDP predictably alters sensory tuning in vitro and is mediated by NMDA receptors. However, the change in synaptic responses induced by sensory stimulation in vivo did not adhere to the direction predicted by the STDP observed in vitro. Further analysis suggests that this difference is influenced by polysynaptic activity, including inhibitory interneurons. Our findings suggest that STDP rules operating at identified synapses may not drive predictable changes in sensory responses at the circuit level.NEW & NOTEWORTHYWe replicated behaviorally relevant temporal patterns of synaptic activity in vitro and used the same patterns during sensory stimulation in vivo. There was a Hebbian spike timing-dependent plasticity (STDP) pattern in vitro, but sensory responses in vivo did not shift according to STDP predictions. Analysis suggests that this disparity is influenced by differences in polysynaptic activity, including inhibitory interneurons. These results suggest that STDP rules at synapses in vitro do not necessarily apply to circuits in vivo.Listen to this article’s corresponding podcast at https://jneurophysiol.podbean.com/e/jnp-micro-podcasts-four-questions-adalee-j-lube-and-bruce-a-carlson/.