The RNA-binding proteomes from yeast to man harbour conserved enigmRBPs.
The RNA-binding proteomes from yeast to man harbour conserved enigmRBPs.
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DOI：
10.1038/ncomms10127
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发表时间：
2015-12-03
影响因子：
16.6
通讯作者：
Hentze MW
中科院分区：
文献类型：
--
作者：
Beckmann BM;Horos R;Fischer B;Castello A;Eichelbaum K;Alleaume AM;Schwarzl T;Curk T;Foehr S;Huber W;Krijgsveld J;Hentze MW
RNA结合蛋白（RBP）具有广泛的生物学功能。为了探索RBP在真核生物进化中的范围，我们确定了酵母酿酒酵母的体内RBP库，并从酵母中鉴定了678个RBP，另外从人肝细胞HuH-7细胞中鉴定了729个RBP。结合分析这些和最近发表的数据集定义的核心RBP剧目保守从酵母到man. Conserved RBP海港定义的重复图案内无序的区域，显示惊人的进化扩张。只有60%的酵母和73%的人类RBP具有分配给RNA生物学或已知传递RNA结合的结构基序的功能，并且许多深入研究的蛋白质令人惊讶地以RBP（称为“enigmRBP”）出现，包括几乎所有的糖酵解酶，指向基因调控和代谢之间的新兴联系。线粒体羟基类固醇脱氢酶（HSD 17 B10）的分析揭示了一个enigmRBP的RNA结合特异性。 RNA结合蛋白（RBP）参与许多生物学功能。在本文中，作者扩展了人类和酵母RNA相互作用组，鉴定了新的和保守的RBP，其中几个RBP没有先前的功能分配给已知介导RNA结合的RNA生物学或结构基序，并提出了RNA作为蛋白质功能调节剂的新作用。
RNA-binding proteins (RBPs) exert a broad range of biological functions. To explore the scope of RBPs across eukaryotic evolution, we determined the in vivo RBP repertoire of the yeast Saccharomyces cerevisiae and identified 678 RBPs from yeast and additionally 729 RBPs from human hepatocytic HuH-7 cells. Combined analyses of these and recently published data sets define the core RBP repertoire conserved from yeast to man. Conserved RBPs harbour defined repetitive motifs within disordered regions, which display striking evolutionary expansion. Only 60% of yeast and 73% of the human RBPs have functions assigned to RNA biology or structural motifs known to convey RNA binding, and many intensively studied proteins surprisingly emerge as RBPs (termed ‘enigmRBPs'), including almost all glycolytic enzymes, pointing to emerging connections between gene regulation and metabolism. Analyses of the mitochondrial hydroxysteroid dehydrogenase (HSD17B10) uncover the RNA-binding specificity of an enigmRBP. RNA-binding proteins (RBPs) are implicated in many biological functions. Here the authors expand the human and yeast RNA interactome identifying new and conserved RBPs, several of which with no prior function assigned to RNA biology or structural motifs known to mediate RNA-binding, and suggesting new roles of RNA as modulators of protein function.