Escherichia coli and Staphylococcus aureus elicit differential innate immune responses following intramammary infection
Escherichia coli and Staphylococcus aureus elicit differential innate immune responses following intramammary infection
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DOI：
10.1128/cdli.11.3.463-472.2004
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发表时间：
2004-05-01
期刊：
影响因子：
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通讯作者：
Rainard, P
中科院分区：
文献类型：
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作者：
Bannerman, DD;Paape, MJ;Rainard, P
金黄色葡萄球菌和大肠杆菌分别是引起临床乳腺炎的革兰氏阳性和革兰氏阴性细菌中最普遍的菌种。先天性免疫系统包括直接宿主防御机制以保护免受感染，并有助于感染性病原体的初始检测和促炎性应答。本研究的目的是研究不同的天然免疫应答对实验性乳房内感染的E。coli和革兰氏阳性菌S.金黄色葡萄球菌。研究了细胞因子应答和可溶性CD 14（sCD 14）和脂多糖结合蛋白（LBP）水平的变化，这两种蛋白有助于宿主识别细菌细胞壁产物。乳房内感染与E. coli或S.金黄色葡萄球菌引起全身性变化，包括产奶量减少、发热反应和LBP急性期合成的诱导。两种细菌感染均导致乳汁中白细胞介素10（IL-1 β）、γ干扰素、IL-12、sCD 14和LBP水平升高。在大肠杆菌感染后的几个时间点检测到高水平的补体裂解产物C5 a和抗炎细胞因子IL-10。coli感染，而S.金黄色葡萄球菌感染在单个时间点引起这些介质的轻微但可检测的增加。IL-8和肿瘤坏死因子α的增加仅在感染E.杆菌总之，这些数据证明了宿主对E. coli和革兰氏阳性菌S.提示对金黄色葡萄球菌的有限细胞因子应答。金黄色葡萄球菌可能有助于该细菌建立慢性乳房内感染的众所周知的能力。
Staphylococcus aureus and Escherichia coli are among the most prevalent species of gram-positive and gram-negative bacteria, respectively, that induce clinical mastitis. The innate immune system comprises the immediate host defense mechanisms to protect against infection and contributes to the initial detection of and proinflammatory response to infectious pathogens. The objective of the present study was to characterize the different innate immune responses to experimental intramammary infection with E. coli and S. aureus during clinical mastitis. The cytokine response and changes in the levels of soluble CD14 (sCD14) and lipopolysaccharide-binding protein (LBP), two proteins that contribute to host recognition of bacterial cell wall products, were studied. Intramammary infection with either E. coli or S. aureus elicited systemic changes, including decreased milk output, a febrile response, and induction of the acute-phase synthesis of LBP. Infection with either bacterium resulted in increased levels of interleukin 10 (IL-1beta), gamma interferon, IL-12, sCD14, and LBP in milk. High levels of the complement cleavage product C5a and the anti-inflammatory cytokine IL-10 were detected at several time points following E. coli infection, whereas S. aureus infection elicited a slight but detectable increase in these mediators at a single time point. Increases in IL-8 and tumor necrosis factor alpha were observed only in quarters infected with E. coli. Together, these data demonstrate the variability of the host innate immune response to E. coli and S. aureus and suggest that the limited cytokine response to S. aureus may contribute to the well-known ability of the bacterium to establish chronic intramammary infection.