In Arabidopsis thaliana, the invertase inhibitors AtC/VIF1 and 2 exhibit distinct target enzyme specificities and expression profiles
In Arabidopsis thaliana, the invertase inhibitors AtC/VIF1 and 2 exhibit distinct target enzyme specificities and expression profiles
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DOI：
10.1016/j.febslet.2004.07.062
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发表时间：
2004-08-27
期刊：
影响因子：
3.5
通讯作者：
Greiner, S
中科院分区：
文献类型：
--
作者：
Link, M;Rausch, T;Greiner, S
植物细胞壁 (CWI) 和液泡转化酶 (VI) 在碳水化合物代谢、应激反应和糖信号传导中发挥重要作用。为了解决抑制剂蛋白（C/VIF，果糖苷酶的细胞壁/液泡抑制剂）对蔗糖酶活性的调节，我们从拟南芥中鉴定了两种 C/VIF。 AtC/VIF1 显示出对 VI 活性的特异性抑制，而 AtC/VIF2 则抑制 CWI 和 VI。表达分析表明，AtC/VIF1 的表达仅限于特定器官，而 AtCIVIF2 在整个植物发育过程中表达较弱。启动子::GUS 转化体证实了两种亚型之间组织/细胞类型特异性表达的显着差异。 AtC/VIF1 T-DNA KO 突变体的生长不受影响，但 VI 活性和己糖含量略有增加。 (C) 2004 年欧洲生化学会联合会。由 Elsevier B.V. 出版。保留所有权利。
Plant cell wall (CWI) and vacuolar invertases (VI) play important roles in carbohydrate metabolism, stress responses and sugar signaling. Addressing the regulation of invertase activities by inhibitor proteins (C/VIF, cell wall/ vacuolar inhibitor of fructosidase), we have identified two C/ VIFs from Arabidopsis thaliana. AtC/VIF1 showed specific inhibition of VI activity, whereas AtC/VIF2 inhibited both, CWI and VI. Expression analysis revealed that expression of AtC/ VIF1 was restricted to specific organs, AtCIVIF2, however, was weakly expressed throughout plant development. Promoter::GUS transformants confirmed pronounced differences of tissue/cell type-specific expression between both isoforms. Growth of an AtC/VIF1 T-DNA KO mutant was unaffected, but VI activity and hexose content were slightly increased. (C) 2004 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.