A near-infrared ratiometric/turn-on fluorescent probe for in vivo imaging of hydrogen peroxide in a murine model of acute inflammation
A near-infrared ratiometric/turn-on fluorescent probe for in vivo imaging of hydrogen peroxide in a murine model of acute inflammation
复制标题
一种近红外比例/开启荧光探针，用于小鼠急性炎症模型中过氧化氢的体内成像。
DOI：
10.1016/j.aca.2018.03.028
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发表时间：
2018-09-18
影响因子：
6.2
通讯作者：
Liu, Yangping
中科院分区：
文献类型：
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作者：
Hou, Jingli;Qian, Meng;Liu, Yangping
由于过氧化氢（H2 O2）在许多疾病的发生和发展中起着重要作用，因此开发用于无创、定量、体内监测的光学探针受到了广泛关注。基于这一需要，本文报道了通过调节染料9 H-1，3-二氯-7-羟基-9，9-二甲基吖啶-2-酮（DDAO）的分子内电荷转移（ICT）过程，合成了H2 O2的近红外荧光探针（AB 1）。探针AB 1表现出大的NIR荧光开启和对（H2 O2）的比率响应，具有高灵敏度和特异性。AB_1的荧光响应与H_2 O_2在1 μ M ~ 100 μ M的较宽浓度范围内呈良好的线性关系，检出限为0.42 μ M。共聚焦显微镜实验表明，AB 1可以比率检测活细胞中的外源性和内源性H2 O2。此外，由于DDAO的近红外发射，该探针还用于成像内源性（H2 O2）从腹腔内的脂多糖诱导的急性炎症的小鼠模型，基于荧光开启模式。这种新的探针显示出巨大的潜力，作为一个可靠的化学工具，研究（H2 O2）相关疾病的发展和进展的活动物。(C)2018 Elsevier B. V.版权所有。
Much attention has been paid to develop optical probes for noninvasive, quantitative, in vivo monitoring of hydrogen peroxide (H2O2) due to its important roles in the initiation and development of numerous diseases. Motivated to meet this need, we herein report the synthesis of a near-infrared (NIR) fluorescent probe (AB1) for (H2O2) by modulating intramolecular charge transfer (ICT) process of the dye 9H-1,3-Dichloro-7-hydroxy-9,9-dimethylacridine-2-one (DDAO). The probe AB1 exhibits both a large NIR fluorescence turn-on and a ratiometric response to (H2O2)with high sensitivity and specificity. The fluorescence response of AB1 has a good linear relationship with H2O2) over a wide concentration range from 1 mu M to 100 mu M, thus affording a detection limit of 0.42 mu M. Confocal microscopic experiments demonstrated that AB1 could ratiometrically detect exogenous and endogenous H2O2 in living cells. Moreover, owing to the NIR emission of DDAO, the probe was also utilized to image endogenous (H2O2) from the peritoneal cavity in a mouse model of lipopolysaccharide-induced acute inflammation, based on the fluorescence turn-on mode. This new probe shows great potential as a reliable chemical tool to study the development and progression of (H2O2)-associated diseases in living animals. (C) 2018 Elsevier B.V. All rights reserved.