Characterization of carcinoembryonic antigen-related antigens in normal adult feces.
Characterization of carcinoembryonic antigen-related antigens in normal adult feces.
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DOI：
10.1111/j.1349-7006.1990.tb02600.x
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发表时间：
1990-05
期刊：
影响因子：
--
通讯作者：
Kuroki M
中科院分区：
文献类型：
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作者：
Matsuoka Y;Matsuo Y;Sugano K;Ohkura H;Kuroki M;Kuroki M
在一天内排出的正常成人粪便（200-250 g）中检测到癌胚抗原（CEA）（或CEA相关抗原）约50-70 mg。10%或更少的抗原在新鲜粪便中可溶（自然溶解抗原），而90%或更多的抗原仍以膜结合形式存在，可通过磷脂酰肌醇特异性磷脂酶C （PI - PLC -溶解抗原）释放。自然溶解抗原和PI - PLC溶解抗原在抗基因上彼此不同，分别与正常粪便抗原(NFA) - 2和CEA相似，这表明到目前为止在癌组织CEA中检测到的“CEA特异性”抗原性不是由于正常组织和恶性组织中的抗原之间的差异，而可能是由于抗原分子羧基端存在糖基肌醇磷酸基团。因此，“CEA特异性”抗原性绝不是癌症特异性的，但这种抗原性似乎对CEA作为肿瘤标志物的临床意义至关重要，因为最近报道的一种能够区分CEA和NFA - 2的检测系统（Kit II）揭示了癌症诊断的许多改进特征。
About 50–70 mg in total of carcinoembryonic antigen (CEA) (or CEA‐related antigens) was detected in normal adult feces evacuated during one day (200–250 g). Ten percent or less of the antigen was found to be in soluble form in fresh feces (naturally solubilized antigen), while 90% or more was still in membrane‐bound form which was releasable with phosphatidylinositol‐specific phospholipase C (PI‐PLC‐solubilized antigen). The naturally solubilized and PI‐PLC‐solubilized antigens are anti‐genically different from each other and similar to normal fecal antigen (NFA)‐2 and CEA, respectively, suggesting that “CEA‐distinctive’antigenicity detected so far in CEA from cancerous tissues is not due to the difference between antigens in normal and malignant tissues but is probably due to the presence of the glycosylinositolphosphate moiety at the carboxyl‐terminus of the antigen molecule. Thus, “CEA‐distinctive’antigenicity is by no means cancer‐specific, but this antigenicity seems to be critical for the clinical significance of CEA as a tumor marker, because an assay system (Kit II) which is able to distinguish CEA from NFA‐2 revealed much improved features in cancer diagnosis as reported recently.