Surface plasmon resonance biosensor based on graphene layer for the detection of waterborne bacteria
Surface plasmon resonance biosensor based on graphene layer for the detection of waterborne bacteria
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DOI：
10.1002/jbio.202200001
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发表时间：
2022-02-07
影响因子：
2.8
通讯作者：
Ramahi, Omar
中科院分区：
文献类型：
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作者：
Daher, Malek G.;Taya, Sofyan A.;Ramahi, Omar
由于水传播细菌给人体带来的风险，在饮用水中识别它们已成为全球关注的问题。本文提出了一种由棱镜、银、石墨烯、亲和层和传感介质组成的高灵敏表面等离子体共振(SPR)生物传感器，用于快速检测水生细菌。首先研究了棱镜/银/传感介质、棱镜/银/亲和层/传感介质、棱镜/银/石墨烯/传感介质和棱镜/银/石墨烯/亲和层/传感介质结构的四种SPR传感器。后一种结构被发现具有最高的敏感性，因此考虑对其进行进一步的研究。然后演示了四种不同的常用棱镜，即N-FK51A、2S2G、SF10和BK7。具有棱镜N-FK51a的结构对应的灵敏度最高，因此考虑对其进行进一步的研究。然后对结构参数进行了优化。该传感器对大肠杆菌的灵敏度约为221.63度/RIU，对霍乱弧菌的灵敏度约为178.12度/RIU，平均为199.87度/RIU。我们相信，拟议的结构将在微生物检测领域打开一个新的窗口。
As a result of the risks that waterborne bacteria bring to the human body, identifying them in drinking water has become a global concern. In this article, a highly sensitive surface plasmon resonance (SPR) biosensor consisting of prism, Ag, graphene, affinity layer and sensing medium is proposed for rapid detection of the waterborne bacteria. Four SPR-based sensors are first studied with the structures prism/Ag/sensing medium, prism/Ag/affinity layer/sensing medium, prism/Ag/graphene/sensing medium, and prism/Ag/graphene/affinity layer/sensing medium. The latter structure is found to have the highest sensitivity so it is considered for further investigations. Four different commonly used prisms are then demonstrated which are N-FK51A, 2S2G, SF10 and BK7. The structure with the prism N-FK51A is found to correspond to the highest sensitivity so it is considered for further investigations. The structure parameters are then optimized. The proposed SPR sensor can achieve high sensitivity of about 221.63 degrees/RIU for Escherichia coli and 178.12 degrees/RIU for Vibrio cholera bacteria with an average value of 199.87 degrees/RIU. We believe that the proposed structure will open a new window in the field of microorganism detections.