Depolarization of cell membrane is associated with an increase in ciliary beat frequency (CBF).
Depolarization of cell membrane is associated with an increase in ciliary beat frequency (CBF).
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细胞膜的去极化与纤毛跳动频率（CBF）的增加有关。
DOI：
10.1006/bbrc.1995.2565
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
Wong,LB
中科院分区：
文献类型：
--
作者：
Mao,H;Wong,LB
We hypothesize that activation of muscarinic cholinergic receptors depolarizes the cell membrane of the mammalian ciliated cells which in turn causes an increase of CBF. To test this hypothesis, a di-8-ANEPPS fluorescence photon counting and nonstationary heterodyne laser light scattering system was developed to measure cell membrane potential (ψ) and CBF in cultured ovine tracheal ciliated cells simultaneously. Carbachol dose dependently depolarized the cell membrane with a corresponding stimulation of CBF. The carbachol induced depolarization of cell membrane and increases of CBF were inhibited by prior application of either atropine or verapamil or amiloride. These novel data suggest that depolarization of the cell membrane and the corresponding stimulation of CBF caused by the activation of muscarinic receptors of the mammalian ciliated cells are dependent on the influx of either extracellular Ca2+or Na+.
