AN ELECTRON MICROSCOPE STUDY OF DEVELOPING BILE CANALICULI IN RAT
AN ELECTRON MICROSCOPE STUDY OF DEVELOPING BILE CANALICULI IN RAT
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DOI：
10.1002/ar.1091510403
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发表时间：
1965-01-01
影响因子：
--
通讯作者：
WOOD, RL
中科院分区：
文献类型：
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作者：
WOOD, RL
用电子显微镜观察了胆小管的发育和管周小体的出现顺序。第一个胆小管出现在胚胎第13天，与相邻肝细胞之间的线性附着区密切相关。这些胆小管只由两个相对的细胞表面组成，中央胆管腔的结构由4-5个相邻细胞包围，经典地描述为哺乳动物肝脏发育的典型特征，直到第16天或第17天才显现出来。扩大的胆管间隙明显地以单独的囊泡或球囊的形式出现，随后伸长成小管和相互连接。本研究的证据支持肝门周围结缔组织的影响下，肝细胞排列的管状通道直接转化为终末胆管的观点。肩周体的发育与肥大的高尔基带有关。出现的顺序是多泡体、微体、典型的溶酶体。这项研究没有证据表明微体与线粒体的分化有关，但出现的时间顺序表明与溶酶体有密切的关系。
The development of bile canaliculi and the sequence of appearance of pericanalicular bodies has been explored by electron microscopy. The first bile canaliculi appear on about the thirteenth embryonic day in close association with linear attachment zones between adjacent hepatic cells. These canaliculi are formed by only two apposed cell surfaces and the configuration of a central biliary lumen bounded by 4-5 contiguous cells described classically as typical for developing mammalian liver is not apparent until day 16 or 17. The enlarged biliary spaces apparently arise as separate vesicles or saccules which subsequently elongate into tubules and interconnect. Evidence obtained in this study supports the concept that terminal bile ductules develop by direct transformation of tubular channels lined by hepatic cells under the influence of periportal connective tissue. Pericanalicular bodies develop in association with an hypertrophied Golgi zone. The sequence of appearance is multivesicular bodies, microbodies, typical lysosomes. There is no evidence from this study that microbodies are related to differentiating mitochondria but the chronology of appearance suggests a close relationship to lysosomes.