K562 - HUMAN ERYTHROLEUKEMIC CELL-LINE
K562 - HUMAN ERYTHROLEUKEMIC CELL-LINE
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DOI：
10.1002/ijc.2910230202
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发表时间：
1979-01-01
影响因子：
6.4
通讯作者：
GAHMBERG, CG
中科院分区：
文献类型：
--
作者：
ANDERSSON, LC;NILSSON, K;GAHMBERG, CG
研究了人白血病细胞系K562的表面膜特性，此前报道K562代表了粒细胞成熟的早期阶段。经半乳糖氧化酶- nab [3H]4标记和平板凝胶电泳得到的K562细胞的表面糖蛋白图谱与正常红细胞有显著的相似之处，但与成髓细胞向粒细胞分化不同阶段的正常细胞和恶性细胞的表面糖蛋白图谱完全不同。免疫荧光和标记膜的免疫沉淀显示，K562细胞在其表面表达主要的红细胞唾液糖蛋白，糖蛋白。由于糖蛋白只存在于人骨髓中的红细胞中，因此K562可能是人红白血病系。
The surface membrane properties of the human leukemic cell line K562, which previously was reported to represent an early stage of granulocyte maturation, were studied. The surface glycoprotein pattern of the K562 cells obtained after galactose oxidase-NaB[3H]4 labeling and slab gel electrophoresis shows striking similarities with that of normal erythrocytes but is completely different from the patterns of normal and malignant cells of various stages of the myeloblast to granulocyte differentiation. The K562 cell expressed the major red cell sialoglycoprotein, glycophorin, on its surface as shown by immunofluorescence and by immunoprecipitation from labeled membrane preparations. As glycophorin is exclusively found on erythroid cells in human bone marrow, the K562 probably is a human erythroleukemic line.