Z-scan fluorescence profile deconvolution of cytosolic and membrane-associated protein populations.
Z-scan fluorescence profile deconvolution of cytosolic and membrane-associated protein populations.
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胞质和膜相关蛋白群体的 Z 扫描荧光图解卷积。
DOI：
10.1016/j.ab.2015.03.030
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发表时间：
2015
影响因子：
2.9
通讯作者：
Mueller,JoachimD
中科院分区：
文献类型：
--
作者：
Smith,ElizabethM;Hennen,Jared;Chen,Yan;Mueller,JoachimD
This study introduces a technique that characterizes the spatial distribution of peripheral membrane proteins that associate reversibly with the plasma membrane. An axial scan through the cell generates a z-scan intensity profile of a fluorescently labeled peripheral membrane protein. This profile is analytically separated into membrane and cytoplasmic components by accounting for both the cell geometry and the point spread function. We experimentally validated the technique and characterized both the resolvability and stability of z-scan measurements. Furthermore, using the cellular brightness of green fluorescent protein, we were able to convert the fluorescence intensities into concentrations at the membrane and in the cytoplasm. We applied the technique to study the translocation of the pleckstrin homology domain of phospholipase C delta 1 labeled with green fluorescent protein on ionomycin treatment. Analysis of the z-scan fluorescence profiles revealed protein-specific cell height changes and allowed for comparison between the observed fluorescence changes and predictions based on the cellular surface area-to-volume ratio. The quantitative capability of z-scan fluorescence profile deconvolution offers opportunities for investigating peripheral membrane proteins in the living cell that were previously not accessible.
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DOI：
10.1073/pnas.92.23.10472
发表时间：
1995-11-07
影响因子：
11.1
作者：
LEMMON, MA;FERGUSON, KM;SCHLESSINGER, J
通讯作者：
SCHLESSINGER, J
影响因子：
64.5
作者：
Honda, A;Nogami, M;Kanaho, Y
通讯作者：
Kanaho, Y
一种新颖的流式细胞术测定法，用于量化蛋白质和膜脂之间的相互作用 这项研究得到了德国研究基金会合作研究中心 638 的支持 发表于 JLR 论文，2009 年 1 月 14 日
影响因子：
6.5
作者：
Temmerman K;Nickel W
通讯作者：
Nickel W
影响因子：
3.3
作者：
Zhao H;Lappalainen P
通讯作者：
Lappalainen P
DOI：
10.1073/pnas.94.25.13661
发表时间：
1997-12-09
影响因子：
11.1
作者：
Pelham, RJ;Wang, YL
通讯作者：
Wang, YL