Effect of Hirsutella sinensis Fungus on the Hypothalamic-Pituitary-Adrenal Axis in Lewis Rats with Kidney-Yang Deficiency Syndrome
Effect of Hirsutella sinensis Fungus on the Hypothalamic-Pituitary-Adrenal Axis in Lewis Rats with Kidney-Yang Deficiency Syndrome
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中华被毛孢菌对肾阳虚证Lewis大鼠下丘脑-垂体-肾上腺轴的影响
DOI：
10.1155/2020/5952612
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发表时间：
2020-05-20
影响因子：
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通讯作者：
Shou, Qiyang
中科院分区：
文献类型：
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作者：
Zhang, Lizong;Lang, Jiali;Shou, Qiyang
肾阳虚证是中医经典证候，主要是由于下丘脑-垂体-肾上腺（HPA）轴受损所致。中国被毛孢菌（Hirsutella sinensis fungus，HSF）是冬虫夏草（Cordyceps sinensis）的人工代用品，在中药中有着广泛的应用。然而，HSF对HPA轴和相应的KYDS的影响和可能的机制尚未被研究。本研究采用刘易斯大鼠作为自发性KYDS模型。给刘易斯大鼠灌胃给予HSF低剂量（1 g/kg）和高剂量（2 g/kg）。采用体重、体温和行为测试（包括握力、旷场和Morris水迷宫（MWM）测试）评估KYDS症状。采用酶联免疫吸附法检测血浆促肾上腺皮质醇（ACTH）、皮质酮（CORT）、促肾上腺皮质激素释放激素（CRH）、环磷酸腺苷（cAMP）、环磷酸鸟苷（cGMP）水平。此外，通过定量实时聚合酶链反应（Q-PCR）检测肿瘤坏死因子α（TNF-α）、干扰素γ（IFN-γ）、白细胞介素10（IL-10）、CRH、糖皮质激素受体（GR）和盐皮质激素受体（MR）的mRNA表达。表明刘易斯大鼠具有KYDS症状，HSF治疗通过增强HPA轴功能来改善这些症状，这通过血清中CRH、ACTH和CORT以及尿中17-OHCS的水平增加来证明。HSF还能显著提高Th 1细胞分泌的TNF-α、IFN-γ和IL-2的表达，这可能促进了与HPA轴功能相关的免疫系统的激活。因此，我们得出结论，HSF可以通过调节HPA轴通过加速免疫系统激活缓解刘易斯大鼠的KYDS症状。
Kidney-yang deficiency syndrome (KYDS) is a classic syndrome in traditional Chinese medicine, which is mainly caused by damage to the hypothalamic-pituitary-adrenal (HPA) axis. Hirsutella sinensis fungus (HSF), an artificial substitute of Cordyceps sinensis, has been widely used in TCM. However, the effects and the possible mechanism of HSF on the HPA axis and corresponding KYDS have not yet been investigated. In this study, Lewis rats were used as a spontaneous KYDS model. HSF was intragastrically administered to the Lewis rats at two doses: low dose (1 g/kg) and high dose (2 g/kg). Body weight, temperature, and behavioral tests including grip strength, open field, and Morris water maze (MWM) tests were used to evaluate the KYDS symptoms. Enzyme-linked immunosorbent assay was used to detect the level of circulating adrenocortisol (ACTH), corticosterone (CORT), corticotropin releasing hormone (CRH), cyclic adenosine monophosphate (cAMP), and cyclic guanosine monophosphate (cGMP). In addition, mRNA expression of tumor necrosis factor alpha (TNF-α), interferon gamma (IFN-γ), interleukin 10 (IL-10), CRH, glucocorticoid receptor (GR), and mineralocorticoid receptor (MR) was detected by quantitative real-time polymerase chain reaction (Q-PCR). The Lewis rats were indicated to have KYDS symptoms and HSF treatment ameliorated these symptoms via enhancement of the HPA axis function, which was evidenced by the increased levels of CRH, ACTH, and CORT in serum and 17-OHCS in urine. HSF also significantly improved the expression of TNF-α, IFN-γ, and IL-2, secreted by Th1 cells, which might accelerate the activation of the immune system related to the HPA axis function. Thus, we conclude that HSF can alleviate KYDS symptoms in Lewis rats by regulating the HPA axis through accelerated immune system activation.